Human EPCR ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human EPCR in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
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CD201, EPCR, PROCR, Endothelial protein C receptor, Activated protein C receptor, Endothelial cell protein C receptor, APC receptor
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Sandwich ELISA - Human EPCR ELISA Kit (AB213774)
Human EPCR ELISA Kit (ab213774) Standard Curve.
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Product details
The Human EPCR Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213774) is designed for the quantitative measurement of activated Human EPCR in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCR has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S18-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EPCR amount of sample captured in plate.
Endothelial protein C receptor (EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.
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Biological function summary
The EPCR is part of the protein C anticoagulant pathway where it acts as a cofactor. EPCR is vital in enhancing the activation of protein C on the endothelial surface reducing coagulation activity. It interacts closely with thrombomodulin and protein C to form this complex preventing excessive thrombus formation and contributing to vascular integrity. Apart from its anticoagulant role EPCR also modulates inflammatory responses supporting its function as a bridge between coagulation and inflammation.
Pathways
EPCR lies within the protein C anticoagulation pathway and the endothelial signaling pathways. Its primary partner in the anticoagulation pathway is APC which degrades activated factors Va and VIIIa providing a check against coagulation. Additionally EPCR’s interaction with APC and thrombomodulin links this protein to the endothelial signaling pathway where it affects cell survival and inflammation regulation. This interaction is essential to maintain hemostatic balance and control vascular responses to inflammatory stimuli.
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