AB303749

Human Eph receptor A3 ELISA Kit

Recombinant
SimpleStep
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Human Eph receptor A3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Eph receptor A3 with a sensitivity of 20.103 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ETK, ETK1, HEK, TYRO4, EPHA3, Ephrin type-A receptor 3, EPH-like kinase 4, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, EK4, hEK4, Human embryo kinase, Eph-like tyrosine kinase 1

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Sandwich ELISA - Human Eph receptor A3 ELISA Kit (AB303749)

sELISA

Supplier Data

Sandwich ELISA - Human Eph receptor A3 ELISA Kit (AB303749)

Interpolated concentrations of human Eph receptor A3 in 22Rv1 cell extract.

Interpolated concentration of native Eph receptor A3 was measured in duplicate at different sample concentrations. Undiluted samples are 125 μg/mL 22Rv1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Eph receptor A3 ELISA Kit (AB303749)

Example of human Eph receptor A3 standard curve in 1X Cell Extraction Buffer PTR.

Example of human Eph receptor A3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 20.103 pg/mL

Range

54.688 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Eph receptor A3 SimpleStep ELISA® kit (ab303749) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Eph receptor A3 protein in cell extracts. Quantitate Human Eph receptor A3 with 20.103 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.86" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "84 - 108 %", "average": "= 93" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303749-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303749-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303749-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303749-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303749-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303749-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB303749-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Eph receptor A3 Capture Antibody", "number":"AB303749-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB303749-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Eph receptor A3 Lyophilized Recombinant Protein", "number":"AB303749-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Eph receptor A3 Detector Antibody", "number":"AB303749-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB303749-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor A3 also known as EphA3 is a member of the ephrin receptor subfamily of protein-tyrosine kinases. This receptor with a mass of approximately 120 kDa plays a role in cell-cell communication mainly by interacting with ephrin-A ligands. EphA3 is expressed in various tissues including the nervous system developing tissues and some adult tissues like the lungs and prostate. Its localization varies with the developmental stage and tissue type reflecting its role in numerous processes.

Biological function summary

EphA3 influences cellular organization movement and positioning through its involvement in contact-dependent signaling. It acts as part of a larger receptor-ligand complex which mediates bidirectional signaling between cells. This interaction generally leads to changes in the cytoskeleton impacting cell shape and locomotion. EphA3’s signaling can regulate developmental processes such as axon guidance angiogenesis and tissue boundary formation.

Pathways

EphA3 modulates various important signaling pathways such as the PI3K/AKT pathway and the Rho family GTPase pathway. It interacts with proteins like Ephrin-A5 and other Eph receptors coordinating complex cellular signaling networks. These interactions contribute to the regulation of cell proliferation survival and migration influencing tissue patterning and organ development.

EphA3 has associations with cancer and neurological disorders. It has ties to various cancers like leukemia where it is implicated in proliferation and migration of malignant cells. In the context of neurological conditions EphA3 plays a role in neurodevelopmental disorders. In these diseases connections with other ephrin receptors and proteins involved in cell signaling become critical as they influence pathogenic pathways.

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Product protocols

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Target data

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (PubMed : 11870224, PubMed : 12794130). Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5 (By similarity). Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration (PubMed : 11870224). Also activated by EFNA1, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development (PubMed : 12794130). Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development (By similarity).

See full target information EPHA3

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Eph receptor A3 ELISA Kit

Sandwich ELISA - Human Eph receptor A3 ELISA Kit (AB303749)

Sandwich ELISA - Human Eph receptor A3 ELISA Kit (AB303749)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Proteins & Peptides

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise