Human Eph receptor B3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Eph receptor B3 in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.8 - 400 ng/mL
- Sensitivity
- = 1.8 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
Alternative names
ETK2, HEK2, TYRO6, EPHB3, Ephrin type-B receptor 3, EPH-like tyrosine kinase 2, Embryonic kinase 2, Tyrosine-protein kinase TYRO6, EPH-like kinase 2, EK2, hEK2
What's included?
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Human Eph receptor B3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Eph receptor B3 in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.8 - 400 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.8 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 87.97 | Range 70 - 112 % |
Sample type Plasma | Average % = 75.1 | Range 69 - 90 % |
Sample type Cell culture supernatant | Average % = 97.07 | Range 84 - 121 % |
Sample type Cell culture media | Average % = 97.07 | Range 84 - 121 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Eph receptor B3 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Eph receptor B3 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Eph receptor B3 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Eph receptor B3 also known as Ephrin type-B receptor 3 or EphB3 is a member of the Eph receptor family. These receptors are important elements in receptor tyrosine kinases notable for their involvement in numerous cellular processes. EphB3 has a molecular mass of approximately 110 kDa. EphB3 is predominantly expressed in the brain but also found in lungs intestines and spinal cord. Its expression pattern hints at its diverse roles in various organ systems.
- Biological function summary
Eph receptor B3 plays important roles in cellular communication influencing cell adhesion migration and differentiation. It often acts by forming receptor complexes with its ligands the ephrins. These interactions initiate bidirectional signaling which regulates the positioning of cells in developing tissues. EphB3 is important in guiding axon pathfinding in neurons and vascular patterning during development.
- Pathways
Eph receptor B3 signaling influences the Rho family GTPase pathway. This pathway is involved in cytoskeletal dynamics and cellular morphology changes. EphB3 also partakes in the MAPK/ERK pathway affecting cell proliferation and survival. Through the MAPK pathway EphB3 is linked with related proteins like EphA2 and EphB2 which share overlapping functions in cellular development and differentiation.
- Associated diseases and disorders
Eph receptor B3 has connections to cancer progression and neurological disorders. Changes in EphB3 expression levels have associations with colorectal cancer affecting cell invasion and metastasis. Additionally altered EphB3 signaling is reported in Alzheimer's disease where it seems to interact with the Amyloid precursor protein (APP) contributing to neurodegenerative processes. These associations highlight EphB3's importance in understanding and potentially targeting these conditions.
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1 product image
Sandwich ELISA - Human Eph receptor B3 ELISA Kit (ab309443)
Example of standard curve using ab309443. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
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