Human Ephrin A4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Ephrin A4 in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.35 - 320 ng/mL
- Sensitivity
- = 1.35 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.
Alternative names
EPLG4, LERK4, EFNA4, Ephrin-A4, EPH-related receptor tyrosine kinase ligand 4, LERK-4
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Human Ephrin A4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Ephrin A4 in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.35 - 320 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.35 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Plasma | Average % = 85.47 | Range 70 - 111 % |
Sample type Serum | Average % = 100.6 | Range 82 - 123 % |
Sample type Cell culture media | Average % = 123.1 | Range 118 - 131 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Ephrin A4 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ephrin A4 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Ephrin A4 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ephrin A4 also known as EFNA4 is part of the ephrin family of proteins. It interacts with Eph receptors and primarily functions as a ligand—a molecule that binds to another to form a complex—within this signaling system. Ephrin A4 is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage and its molecular mass is approximately 21 kDa. The expression of Ephrin A4 can be found in various tissues including the brain lungs and kidneys indicating its diverse roles in bodily functions.
- Biological function summary
Ephrin A4 plays significant roles in the development and function of the nervous system. It works within the ephrin/Eph receptor signaling system impacting processes such as cell positioning and tissue boundary formation. Ephrin A4 is not an isolated component; it acts as part of a complex with Eph receptors influencing cell adhesion and shape. These interactions guide the cellular processes required for proper embryonic development.
- Pathways
Ephrin A4 is involved in the ephrin signaling pathway and the MAPK/ERK pathway. These pathways regulate cell proliferation differentiation and migration which are essential for developmental processes. Ephrin A4 interacts with EphA receptors influencing these signaling cascades. This connection shows its significant influence on various cellular processes including those relevant in neural patterning.
- Associated diseases and disorders
Ephrin A4 has associations with certain cancers and neurodegenerative illnesses. In cancer particularly breast cancer Ephrin A4 dysregulation links to uncontrolled cell proliferation and migration often in connection with EphA2 receptor overexpression. Furthermore in neurodegenerative conditions like Alzheimer's disease altered ephrin signaling involves the degradation of neural structures implicating interactions with EphB2. These associations underline the importance of Ephrin A4 in maintaining cellular and tissue health.
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1 product image
Sandwich ELISA - Human Ephrin A4 ELISA Kit (ab309399)
Example of standard curve using ab309399. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
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