JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB309399

Human Ephrin A4 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Ephrin A4 ELISA Kit is a Sandwich ELISA for the measurement of Human Ephrin A4 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

EPLG4, LERK4, EFNA4, Ephrin-A4, EPH-related receptor tyrosine kinase ligand 4, LERK-4

1 Images
Sandwich ELISA - Human Ephrin A4 ELISA Kit (AB309399)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ephrin A4 ELISA Kit (AB309399)

Example of standard curve using ab309399. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.35 ng/mL

Range

1.35 - 320 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB232711

Anti-Ephrin A4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A4 antibody (AB232711)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Ephrin A4 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ephrin A4 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Ephrin A4 coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Plasma", "range": "70 - 111 %", "average": "= 85.47" }, { "sample": "Serum", "range": "82 - 123 %", "average": "= 100.6" }, { "sample": "Cell culture media", "range": "118 - 131 %", "average": "= 123.1" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309399-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human Ephrin-A4/EFNA4 Protein Standard", "number":"AB309399-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309399-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309399-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309399-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309399-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Ephrin-A4/EFNA4 Detection Antibody", "number":"AB309399-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB309399-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Ephrin-A4/EFNA4 Antibody-coated ELISA Plate", "number":"AB309399-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin A4 also known as EFNA4 is part of the ephrin family of proteins. It interacts with Eph receptors and primarily functions as a ligand—a molecule that binds to another to form a complex—within this signaling system. Ephrin A4 is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage and its molecular mass is approximately 21 kDa. The expression of Ephrin A4 can be found in various tissues including the brain lungs and kidneys indicating its diverse roles in bodily functions.
Biological function summary

Ephrin A4 plays significant roles in the development and function of the nervous system. It works within the ephrin/Eph receptor signaling system impacting processes such as cell positioning and tissue boundary formation. Ephrin A4 is not an isolated component; it acts as part of a complex with Eph receptors influencing cell adhesion and shape. These interactions guide the cellular processes required for proper embryonic development.

Pathways

Ephrin A4 is involved in the ephrin signaling pathway and the MAPK/ERK pathway. These pathways regulate cell proliferation differentiation and migration which are essential for developmental processes. Ephrin A4 interacts with EphA receptors influencing these signaling cascades. This connection shows its significant influence on various cellular processes including those relevant in neural patterning.

Ephrin A4 has associations with certain cancers and neurodegenerative illnesses. In cancer particularly breast cancer Ephrin A4 dysregulation links to uncontrolled cell proliferation and migration often in connection with EphA2 receptor overexpression. Furthermore in neurodegenerative conditions like Alzheimer's disease altered ephrin signaling involves the degradation of neural structures implicating interactions with EphB2. These associations underline the importance of Ephrin A4 in maintaining cellular and tissue health.
style
left-column

Product protocols

style
left-column

Target data

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.
See full target information EFNA4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com