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AB213775

Human Epiregulin ELISA Kit

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Human Epiregulin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Epiregulin in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Proepiregulin, EREG

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Sandwich ELISA - Human Epiregulin ELISA Kit (AB213775)
  • sELISA

Supplier Data

Sandwich ELISA - Human Epiregulin ELISA Kit (AB213775)

Human Epiregulin ELISA Kit (ab213775) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

3.9 - 250 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Epiregulin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213775) is designed for the quantitative measurement of Human Epiregulin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Epiregulin has been pre-coated onto 96-well plates. Standards (E.coli; V60-L108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Epiregulin amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "56 pg/mL", "standardDeviation": "4.48", "coefficientOfVariability": "= 8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "105 pg/mL", "standardDeviation": "6.4", "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "13 pg/mL", "standardDeviation": "0.78", "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "113 pg/mL", "standardDeviation": "6.1", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "12 pg/mL", "standardDeviation": "0.94", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "57 pg/mL", "standardDeviation": "4.21", "coefficientOfVariability": "= 7.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213775-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213775-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human Epiregulin antibody", "number":"AB213775-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Epiregulin coated Microplate (12 x 8 wells)", "number":"AB213775-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213775-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213775-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213775-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213775-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human Epiregulin Standard", "number":"AB213775-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213775-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213775-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213775-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EREG protein also known as epiregulin is part of the epidermal growth factor (EGF) family. It is a small polypeptide with a molecular weight of approximately 19 kDa. EREG is expressed in various tissues including the liver kidney and colon. It interacts with EGF receptors (EGFR) activating them through dimerization which then triggers downstream signaling processes.
Biological function summary

Epiregulin plays a significant role in cell proliferation migration and differentiation. It is not part of a larger complex but functions by binding to EGFR to exert its effects. Its interaction with EGFR influences numerous cellular processes important for tissue repair and development making EREG an important player in physiological and pathological conditions.

Pathways

Epiregulin is tied into the EGFR signaling pathway and the MAPK pathway. It works together with other growth factors like transforming growth factor-alpha (TGF-α) in these pathways. Through EGFR activation EREG influences cellular responses that are critical for growth and survival linking it to broader biological functions involving MAPK signaling cascades.

Epiregulin links to cancer progression and inflammatory diseases. In some cancers EREG overexpression correlates with aggressive tumor growth and poor prognosis. In inflammatory conditions it can modulate responses impacting diseases such as psoriasis by interacting with proteins like EGFR. Understanding EREG's roles provides insights into therapeutic strategies targeting EGFR-related pathways.
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Product protocols

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Target data

Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed : 9419975). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed : 24631357).
See full target information Proepiregulin
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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