AB277077

Human Epiregulin ELISA Kit (EREG)

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Human Epiregulin ELISA Kit (EREG) is a single-wash 90-min Simplestep used to quantify Human Epiregulin (EREG) with a sensitivity of 5.803 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Proepiregulin, EREG

3 Images

Sandwich ELISA - Human Epiregulin ELISA Kit (EREG) (AB277077)

sELISA

Supplier Data

Sandwich ELISA - Human Epiregulin ELISA Kit (EREG) (AB277077)

Serum from eight individual healthy Epiregulin female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Epiregulin concentration was determined to be 23.5 pg/mL. Seven samples tested below the detection limit on the assay.

sELISA

Supplier Data

Sandwich ELISA - Human Epiregulin ELISA Kit (EREG) (AB277077)

Example of human Epiregulin standard curve in Sample Diluent NS.

The Epiregulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Epiregulin ELISA Kit (EREG) (AB277077)

Interpolated concentrations of native Epiregulin in human U-87 cell supernatant.

The concentrations of Epiregulin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : U-87 cell supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 244.1 pg/mL in U-87 cell supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.803 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

style

left-column

Product details

Human Epiregulin ELISA kit (ab277077) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Epiregulin protein in human serum and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Epiregulin with 5.8 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Epiregulin is a secreted peptide hormone encoded by the gene EREG. Epiregulin is a member of the epidermal growth factor family. Epiregulin is expressed as a type I membrane pro-form, proepiregulin, and epiregulin is catalytic cleaved to a 46- amino acid soluble form by disintegrin and metalloproteinase (ADAM). Epiregulin functions as a ligand of epidermal growth factor receptor (EGFR)and most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. Epiregulin contributes to inflammation, wound healing, tissue repair, and oocyte maturation.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 108 %", "average": "= 102" }, { "sample": "Serum", "range": "80 - 86 %", "average": "= 83" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277077-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277077-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277077-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277077-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277077-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277077-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Epiregulin (EREG) Capture Antibody", "number":"AB277077-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Epiregulin (EREG) Lyophilized Recombinant Protein", "number":"AB277077-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB277077-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Epiregulin (EREG) Detector Antibody", "number":"AB277077-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277077-CMP05", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epiregulin also known as EREG is a member of the epidermal growth factor (EGF) family. It is a glycoprotein with a molecular weight of approximately 19 kDa. The protein is secreted and functions as a ligand for the EGF receptor (EGFR) and to a lesser extent for ErbB4 known as HER4 in humans. Epiregulin is expressed in a variety of tissues including placenta kidney and skin as well as within certain tumor cells. The expression can be regulated by signaling pathways involved in cellular growth and differentiation.

Biological function summary

Epiregulin participates in cellular processes such as proliferation differentiation and migration. It functions by binding to the EGFR initiating a cascade of downstream signaling events. As part of the EGF family it does not form part of a larger complex but it influences the activity of receptor dimers once bound. Its activity varies depending on the cellular context aiding in processes like tissue repair and embryonic development through its interaction with receptors and activation of signaling pathways.

Pathways

Epiregulin plays a significant role in the MAPK/ERK and PI3K/Akt signaling pathways both critical in regulating cell growth and survival. These pathways influence critical processes like cell cycle progression and apoptosis where epiregulin functions in coordination with other EGF family proteins such as TGF-alpha and amphiregulin. This protein assists in maintaining cellular homeostasis by modulating these pathways which are key to responding to environmental stimuli and cellular stress.

Epiregulin has associations with certain cancers such as colorectal and breast cancer. The protein often shows elevated expression levels in tumor samples implicating it in tumorigenesis. Its binding interaction with EGFR plays an important role in cancer cell survival and proliferation. Additionally epiregulin bears a connection to inflammatory diseases where its expression can exacerbate conditions like psoriasis through its impact on epidermal cells. In both cancer and inflammatory conditions the interplay between epiregulin and other related proteins like EGFR and ERBB2 influences disease progression and severity.

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

style

left-column

Target data

Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed : 9419975). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed : 24631357).

See full target information Proepiregulin

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science immunology 7:eabq6691 PubMed36490328

2022

Epiregulin is a dendritic cell-derived EGFR ligand that maintains skin and lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ian D Odell,Holly Steach,Stephen B Gauld,Lauren Reinke-Breen,Jozsef Karman,Tracy L Carr,Joseph B Wetter,Lucy Phillips,Monique Hinchcliff,Richard A Flavell

Scientific reports 11:11799 PubMed34083693

2021

BET inhibitor suppresses migration of human hepatocellular carcinoma by inhibiting SMARCA4.

Applications

Unspecified application

Species

Unspecified reactive species

Hae In Choi,Ga Yeong An,Mina Baek,Eunyoung Yoo,Jin Choul Chai,Young Seek Lee,Kyoung Hwa Jung,Young Gyu Chai

View all publications

style

left-column

style

left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

style

left-column

websiteProtocolBooklet

style

right-column

style

bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com