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Human EPO-R ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human EPO-R in Human in Serum, Plasma, Cell culture supernatant samples.

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Sandwich ELISA - Human EPO-R ELISA Kit (AB314728), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

180 - 12000 pg/mL

Sensitivity

= 180 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed:10388848, PubMed:2163695, PubMed:2163696, PubMed:8662939, PubMed:9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed:11756159). May also activate the LYN tyrosine kinase (By similarity).Isoform EPOR-TActs as a dominant-negative receptor of EPOR-mediated signaling.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
300X HRP-Streptavidin
1 x 200 µL
5X Assay Diluent B (Item E)
1 x 15 mL
5X Assay Diluent D (Item K)
1 x 15 mL
Biotinylated Human EPO-R Detection Antibody
2 x 1 Vial
Human EPO-R Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human EPO-R Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Human EPO-R ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human EPO-R in Human in Serum, Plasma, Cell culture supernatant samples.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

180 - 12000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 180 pg/mL

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 85.4

Range

70 - 112 %

Sample type

Plasma

Average %

= 95.4

Range

69 - 126 %

Sample type

Cell culture media

Average %

= 96.49

Range

83 - 109 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human EPO-R ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human EPO-R in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human EPO-R coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, IFN-g, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1a, MIP-1 b, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF1 alpha, TARC, TGF-b, TIMP-1, TIMP-2, TNF-a, TNF-b, TPO, VEGF.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Erythropoietin receptor (EPO-R) also known as EPOR plays an important role in erythropoiesis by mediating the effects of erythropoietin (EPO). EPO-R is a member of the cytokine receptor family with a mass of approximately 55 kDa. This receptor can primarily be found on the surface of erythroid progenitor cells in the bone marrow. Its presence is important for the final differentiation of these cells into mature erythrocytes.

Biological function summary

EPO-R functions by dimerizing upon EPO binding subsequently activating intracellular signaling pathways. It is a component of a larger signaling complex that recruits and activates various molecules initiating a cascade of events critical for cell survival and proliferation. This receptor signaling prevents apoptosis in erythroid progenitor cells promoting their growth and differentiation into red blood cells.

Pathways

EPO-R's activity falls within the JAK-STAT and PI3K-AKT signaling pathways. These pathways are pivotal in regulating hematopoiesis and cell survival. JAK2 acts as a direct partner to EPO-R and transduces the signal downstream while molecules like STAT5 get phosphorylated and mediate transcription of target genes. The PI3K-AKT pathway also contributes to the anti-apoptotic effect essential for erythrocyte development.

Associated diseases and disorders

Altered EPO-R signaling associates with anemia of chronic disease and polycythemia vera. Anemic conditions often show reduced sensitivity or expression of EPO-R while mutations leading to EPO-R hypersensitivity link to polycythemia vera. Dysregulation of related proteins such as JAK2 also contributes to these conditions indicating the interconnected roles of these proteins in blood-related diseases.

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1 product image

  • Sandwich ELISA - Human EPO-R ELISA Kit (ab314728), expandable thumbnail

    Sandwich ELISA - Human EPO-R ELISA Kit (ab314728)

    Example of standard curve using ab314728. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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