JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108732

Human Anti-Epstein Barr virus IgM ELISA Kit (EBV-VCA)

Be the first to review this product! Submit a review

|

(3 Publications)

Human Anti-Epstein Barr virus IgM ELISA Kit (EBV-VCA) is an indirect ELISA for the qualitative detection of IgM class antibodies against Epstein Barr virus (EBV-VCA) in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's anti-Epstein Barr virus (EBV-VCA) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Epstein Barr virus in Human serum and plasma.

A 96-well plate has been precoated with Epstein Barr virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Epstein Barr virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Epstein Barr virus IgM sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 25, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108732-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Epstein-Barr Virus IgM Positive Control", "number":"AB108732-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"Epstein-Barr Virus IgM Negative Control", "number":"AB108732-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Epstein Barr virus anti-IgM HRP Conjugate", "number":"AB108732-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108732-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Epstein Barr virus (IgM) Coated Microplate (12 x 8 wells)", "number":"AB108732-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108732-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108732-CMP11", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB108732-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108732-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108732-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"Epstein-Barr-Virus IgM Cut-off Control", "number":"AB108732-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target Epstein-Barr Virus (VCA) IgM also known as EBV IgM VCA IgM or EBV capsid IgM serves as an early serological marker for Epstein-Barr Virus infections. This immunoglobulin is directed against the viral capsid antigen (VCA) and typically appears during the acute phase of infection. EBV VCA IgM is expressed on the surface of B cells and in the bloodstream. Though its molecular mass can vary IgM generally weighs around 950 kDa consisting of heavy and light chains forming a pentamer structure.
Biological function summary

EBV VCA IgM is integral to the body's initial immune response against the Epstein-Barr Virus. It is not part of a larger protein complex but operates independently as an antibody that detects viral antigens. It binds to the Epstein-Barr early antigens facilitating neutralization and clearance of the virus. Its presence indicates a recent EBV infection and is important for diagnosing infectious mononucleosis commonly called mono.

Pathways

EBV VCA IgM is involved in the immune response pathways particularly humoral immune processes. This antibody participates in the activation of the complement cascade enhancing opsonization and phagocytosis of the virus by immune cells. It closely relates to another antibody EBV IgG which appears later in the infection cycle indicating past exposure and ongoing immune response maintenance.

EBV VCA IgM is strongly associated with infectious mononucleosis and can also play a role in certain autoimmune conditions triggered by EBV infection. The early presence of this IgM antibody often precedes the development of post-transplant lymphoproliferative disorder in immunocompromised individuals. EBV VCA IgM interactions with B cells contribute to the pathogenesis pointing towards its importance in viral-mediated disease progression.
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Open forum infectious diseases 11:ofae693 PubMed39703789

2024

Herpesvirus Antibodies Are Correlated With Greater Expression of p16 in the T Cells of Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Noppert,Kathleen Wragg,Chihua Li,Kate Duchowny,Lona Mody,Allison E Aiello,Linda Nyquist,Martin O'Brien,Raymond Yung,Daniel Goldstein

Journal of inflammation (London, England) 21:30 PubMed39135051

2024

Evidence of aberrant anti-epstein-barr virus antibody response, though no viral reactivation, in people with post-stroke fatigue.

Applications

Unspecified application

Species

Unspecified reactive species

Isobel C Mouat,Li Zhu,Alperen Aslan,Barry W McColl,Stuart M Allan,Craig J Smith,Marion S Buckwalter,Laura McCulloch

Journal of immunology research 2022:4363927 PubMed36405008

2022

Low Level of Advanced Glycation End Products in Serum of Patients with Allergic Rhinitis and Chronic Epstein-Barr Virus Infection at Different Stages of Virus Persistence.

Applications

Unspecified application

Species

Unspecified reactive species

Svitlana Zubchenko,Iryna Kril,Halyna Potemkina,Anna Havrylyuk,Aleksandra Kuzan,Andrzej Gamian,Valentyna V Chopyak
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB108730

Human Anti-Epstein Barr virus IgG ELISA Kit (EBV-VCA)

  • 5
  • 1
Assay Kits

AB108731

Human Anti-Epstein Barr virus IgG ELISA Kit (EBV-EBNA)

  • 0
  • 0
Assay Kits

AB108782

Human Anti-Varicella-Zoster virus IgG ELISA Kit (VZV)

  • 0
  • 0
Assay Kits

AB108725

Human Anti-Cytomegalovirus IgM ELISA Kit (CMV)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com