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Human ERAP2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ERAP2 in Human in Plasma, Cell culture supernatant, Serum samples.

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Images

ELISA - Human ERAP2 ELISA Kit (AB277422), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1.229 - 300 ng/mL

Sensitivity

= 1.23 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
300X HRP-Streptavidin Concentrate
1 x 200 µL
5X Assay Diluent
1 x 15 mL
Anti-Human ERAP2 coated Microplate
1 x 1 Unit
Biotinylated Anti-Human ERAP2 Antibody
2 x 1 Vial
Human ERAP2 standard protein (Lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human ERAP2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ERAP2 in Human in Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1.229 - 300 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 1.23 ng/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 12

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 94.06

Range

68 - 124 %

Sample type

Plasma

Average %

= 82.22

Range

68 - 97 %

Sample type

Cell culture media

Average %

= 108

Range

72 - 140 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human ERAP2 ELISA Kit (ab277422) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ERAP2 in serum, plasma and cell culture supernatants

This assay employs an antibody specific for Human ERAP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ERAP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ERAP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ERAP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ERAP2 protein also known as endoplasmic reticulum aminopeptidase 2 plays an important mechanical role in the cell. It belongs to the M1 zinc metallopeptidase family and has a molecular weight of about 110 kDa. ERAP2 is widely expressed in a range of tissues with notable expression in immune-related organs such as the spleen and lymph nodes. It functions to trim peptides in the endoplasmic reticulum preparing them for presentation by major histocompatibility complex (MHC) class I molecules.

Biological function summary

The trimming activity of ERAP2 assists the immune system in recognizing antigens. It works closely with other components of the antigen processing machinery including the proteasome and transporter associated with antigen processing (TAP). ERAP2 can form a functional complex with ERAP1 another aminopeptidase enhancing the efficiency of peptide processing. The interworking of these proteins is vital for maintaining the diversity and specificity of peptides generated for the immune defense.

Pathways

The ERAP2 protein is involved in the antigen processing and presentation pathway a critical component of the body's adaptive immune response. This pathway ensures foreign or aberrant peptides are presented to CD8+ T cells triggering an immune response. Related proteins such as ERAP1 and TAP collaborate with ERAP2 within this pathway highlighting an interconnected mechanism for immune surveillance and activity.

Associated diseases and disorders

ERAP2 has associations with autoimmune diseases and inflammatory disorders. Variants and dysregulation of ERAP2 expression have links to ankylosing spondylitis a type of arthritis causing inflammation in the spine's joints and ligaments. Similarly altered ERAP2 function is implicated in inflammatory bowel disease. The interplay with ERAP1 is significant here as both proteins contribute to the altered antigen processing observed in these disorders.

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1 product image

  • ELISA - Human ERAP2 ELISA Kit (ab277422), expandable thumbnail

    ELISA - Human ERAP2 ELISA Kit (ab277422)

    These standard curves are for demonstration only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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