Human ERAP2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ERAP2 in Human in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
1.229 - 300 ng/mL
= 1.23 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.
LRAP, ERAP2, Endoplasmic reticulum aminopeptidase 2, Leukocyte-derived arginine aminopeptidase, L-RAP
Human ERAP2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ERAP2 in Human in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
1.229 - 300 ng/mL
Pre-coated microplate (12 x 8 well strips)
= 1.23 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 94.06 | Range 68 - 124 % |
Sample type Plasma | Average % = 82.22 | Range 68 - 97 % |
Sample type Cell culture media | Average % = 108 | Range 72 - 140 % |
Blue Ice
-20°C
-20°C
-20°C
Human ERAP2 ELISA Kit (ab277422) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ERAP2 in serum, plasma and cell culture supernatants
This assay employs an antibody specific for Human ERAP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ERAP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ERAP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ERAP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This supplementary information is collated from multiple sources and compiled automatically.
The ERAP2 protein also known as endoplasmic reticulum aminopeptidase 2 plays an important mechanical role in the cell. It belongs to the M1 zinc metallopeptidase family and has a molecular weight of about 110 kDa. ERAP2 is widely expressed in a range of tissues with notable expression in immune-related organs such as the spleen and lymph nodes. It functions to trim peptides in the endoplasmic reticulum preparing them for presentation by major histocompatibility complex (MHC) class I molecules.
The trimming activity of ERAP2 assists the immune system in recognizing antigens. It works closely with other components of the antigen processing machinery including the proteasome and transporter associated with antigen processing (TAP). ERAP2 can form a functional complex with ERAP1 another aminopeptidase enhancing the efficiency of peptide processing. The interworking of these proteins is vital for maintaining the diversity and specificity of peptides generated for the immune defense.
The ERAP2 protein is involved in the antigen processing and presentation pathway a critical component of the body's adaptive immune response. This pathway ensures foreign or aberrant peptides are presented to CD8+ T cells triggering an immune response. Related proteins such as ERAP1 and TAP collaborate with ERAP2 within this pathway highlighting an interconnected mechanism for immune surveillance and activity.
ERAP2 has associations with autoimmune diseases and inflammatory disorders. Variants and dysregulation of ERAP2 expression have links to ankylosing spondylitis a type of arthritis causing inflammation in the spine's joints and ligaments. Similarly altered ERAP2 function is implicated in inflammatory bowel disease. The interplay with ERAP1 is significant here as both proteins contribute to the altered antigen processing observed in these disorders.
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These standard curves are for demonstration only. A standard curve must be run with each assay.
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