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AB277422

Human ERAP2 ELISA Kit

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Human ERAP2 ELISA Kit is a Sandwich ELISA for the measurement of Human ERAP2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

LRAP, ERAP2, Endoplasmic reticulum aminopeptidase 2, Leukocyte-derived arginine aminopeptidase, L-RAP

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ELISA - Human ERAP2 ELISA Kit (AB277422)
  • ELISA

Supplier Data

ELISA - Human ERAP2 ELISA Kit (AB277422)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.23 ng/mL

Range

1.229 - 300 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB313567

Anti-ERAP2 antibody [EPR26475-10]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (AB313567)

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Proteins & Peptides

AB163795

Recombinant Human ERAP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ERAP2 protein (GST tag N-Terminus) (AB163795)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ERAP2 ELISA Kit (ab277422) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ERAP2 in serum, plasma and cell culture supernatants

This assay employs an antibody specific for Human ERAP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ERAP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ERAP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ERAP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "68 - 124 %", "average": "= 94.06" }, { "sample": "Plasma", "range": "68 - 97 %", "average": "= 82.22" }, { "sample": "Cell culture media", "range": "72 - 140 %", "average": "= 108" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277422-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277422-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277422-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277422-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human ERAP2 coated Microplate", "number":"AB277422-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human ERAP2 Antibody", "number":"AB277422-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB277422-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ERAP2 standard protein (Lyophilized)", "number":"AB277422-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277422-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ERAP2 protein also known as endoplasmic reticulum aminopeptidase 2 plays an important mechanical role in the cell. It belongs to the M1 zinc metallopeptidase family and has a molecular weight of about 110 kDa. ERAP2 is widely expressed in a range of tissues with notable expression in immune-related organs such as the spleen and lymph nodes. It functions to trim peptides in the endoplasmic reticulum preparing them for presentation by major histocompatibility complex (MHC) class I molecules.
Biological function summary

The trimming activity of ERAP2 assists the immune system in recognizing antigens. It works closely with other components of the antigen processing machinery including the proteasome and transporter associated with antigen processing (TAP). ERAP2 can form a functional complex with ERAP1 another aminopeptidase enhancing the efficiency of peptide processing. The interworking of these proteins is vital for maintaining the diversity and specificity of peptides generated for the immune defense.

Pathways

The ERAP2 protein is involved in the antigen processing and presentation pathway a critical component of the body's adaptive immune response. This pathway ensures foreign or aberrant peptides are presented to CD8+ T cells triggering an immune response. Related proteins such as ERAP1 and TAP collaborate with ERAP2 within this pathway highlighting an interconnected mechanism for immune surveillance and activity.

ERAP2 has associations with autoimmune diseases and inflammatory disorders. Variants and dysregulation of ERAP2 expression have links to ankylosing spondylitis a type of arthritis causing inflammation in the spine's joints and ligaments. Similarly altered ERAP2 function is implicated in inflammatory bowel disease. The interplay with ERAP1 is significant here as both proteins contribute to the altered antigen processing observed in these disorders.
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Product protocols

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Target data

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.
See full target information ERAP2
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websiteProtocolBooklet
en
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Product promise

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