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AB283881

Human ErbB2 / HER2 ELISA Kit

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Human ErbB2 / HER2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ErbB2 / HER2 with a sensitivity of 4.95 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

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Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)
  • sELISA

Supplier Data

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

Interpolated concentrations of native ErbB2 / HER2 in human serum and plasma. The concentrations of ErbB2 / HER2 were measured in duplicate, interpolated from the ErbB2 / HER2 standard curve and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, plasma (heparin) 5%, and milk 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ErbB2 / HER2 concentration were determined to be 13.67 ng/mL in serum, 14.20 ng/mL in plasma (citrate), 11.95 ng/mL in plasma (EDTA), 13.51 ng/mL in plasma (heparin), and 1.51 ng/mL in milk.

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)
  • sELISA

Supplier Data

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

Serum from ten individual healthy human female donors was measured in duplicate

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)
  • sELISA

Supplier Data

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

Human ErbB2 competitor curve comparison

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)
  • sELISA

Supplier Data

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

Interpolated concentrations of native ErbB2 / HER2 in human MCF7 cell extract based on a 125 μg/mL extract load and placenta tissue extract based on a 62.5 μg/mL extract load.

The concentrations of ErbB2 / HER2 were measured in duplicate and interpolated from the ErbB2 / HER2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ErbB2 / HER2 concentration was determined to be 2380.97 pg/mL in MCF7 cell extract and 581.18 pg/mL in placenta tissue extract.

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)
  • sELISA

Supplier Data

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

Example of human ErbB2 / HER2 standard curve in Sample Diluent NS.

The ErbB2 / HER2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Milk, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.95 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ErbB2 / HER2 ELISA Kit (ab283881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ErbB2 / HER2 protein in serum, plasma, milk, cell culture media and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ErbB2 / HER2 with 4.95 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human ErbB2 / HER2 (Human Epidermal Growth Factor Receptor 2) is a 185 kDa type I membrane glycoprotein and a member of the ErbB family of tyrosine kinase receptors. The ErbB family serves as receptors for the epidermal growth factor (EGF) family of growth factors. Human ErbB2 / HER2 does not have identified ligands. Instead, ErbB2 heterodimerizes with other members of the ErbB family (ErbB1/EGF R, ErbB3, ErbB4) to form ligand-activated signaling complexes. This kit was designed to target the extracellular region of ErbB2 / HER2. Human ErbB2 / HER2 is involved in cardiovascular homeostasis, communication at the neuromuscular junction, and in the development and progression of cancer.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Milk", "range": "97 - 104 %", "average": "= 100" }, { "sample": "Serum", "range": "84 - 94 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "88 - 104 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "81 - 105 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "80 - 112 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283881-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283881-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283881-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283881-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB283881-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283881-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283881-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283881-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ErbB2 / HER2 Capture Antibody", "number":"AB283881-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ErbB2 / HER2 Lyophilized Recombinant Protein", "number":"AB283881-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB283881-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB283881-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ErbB2 / HER2 Detector Antibody", "number":"AB283881-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
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Product protocols

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Target data

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
See full target information ERBB2
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websiteProtocolBooklet
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Product promise

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