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AB100511

Human ErbB3 / HER3 ELISA Kit

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(1 Publication)

Human ErbB3 / HER3 ELISA Kit is a Sandwich ELISA for the measurement of Human ErbB3 / HER3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

HER3, ERBB3, Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3

2 Images
Sandwich ELISA - Human ErbB3 / HER3 ELISA Kit (AB100511)
  • sELISA

Supplier Data

Sandwich ELISA - Human ErbB3 / HER3 ELISA Kit (AB100511)

TYPICAL STANDARD CURVE - Assay Dilent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Sandwich ELISA - Human ErbB3 / HER3 ELISA Kit (AB100511)
  • sELISA

Supplier Data

Sandwich ELISA - Human ErbB3 / HER3 ELISA Kit (AB100511)

TYPICAL STANDARD CURVE - Assay Diluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 4 pg/mL

Range

3.43 - 2500 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's ErbB3 (Epidermal Factor Growth Factor Receptor 3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB3 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for human ErbB3 coated on a 96-well plate. Standards and samples are pipetted into the wells and ErbB3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ErbB3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "78 - 113 %", "average": "= 100.1" }, { "sample": "Serum", "range": "79 - 111 %", "average": "= 95.12" }, { "sample": "Plasma", "range": "71 - 106 %", "average": "= 88.87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100511-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100511-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100511-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100511-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100511-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ErbB3 Microplate (12 x 8 wells)", "number":"AB100511-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human ErbB3 Standard (lyophilized)", "number":"AB100511-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human ErbB3", "number":"AB100511-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100511-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100511-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.
Biological function summary

HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.

Pathways

HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.

HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.
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Product protocols

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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed : 20682778). May also be activated by CSPG5 (PubMed : 15358134). Involved in the regulation of myeloid cell differentiation (PubMed : 27416908).
See full target information ERBB3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 1655:319-334 PubMed28889394

2017

Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum.

Applications

Unspecified application

Species

Unspecified reactive species

Leandro S D'Abronzo,Chong-Xian Pan,Paramita M Ghosh
View all publications
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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