Human Erythropoietin ELISA Kit (EPO) is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human Erythropoietin (EPO) with a sensitivity of 1 mIU/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Need to design your own assay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.125 - 200 mIU/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1 mIU/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass (PubMed:28283061). Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3 (PubMed:9774108).
Alternative names
Erythropoietin, EPO
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Recommended products
Human Erythropoietin ELISA Kit (EPO) is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human Erythropoietin (EPO) with a sensitivity of 1 mIU/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Need to design your own assay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.125 - 200 mIU/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1 mIU/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 6.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 112 | Range 109 - 114 % |
Sample type Serum | Average % = 99 | Range 98 - 100 % |
Sample type EDTA Plasma | Average % = 104 | Range 96 - 112 % |
Sample type Heparin Plasma | Average % = 91 | Range 90 - 92 % |
Sample type Citrate plasma | Average % = 98 | Range 86 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Erythropoietin ELISA Kit (EPO) ab274397 is a rapid single-wash 90-min ELISA kit to measure Human Erythropoietin (EPO) in cell culture supernatant, Serum and plasma. This SimpleStep ELISA® sensitivity is 1 mIU/mL.
How the assay works
Human Erythropoietin (EPO) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human Erythropoietin. The standard protein in this kit is mature full length human Erythropoietin. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin),and cell culture supernatant sample types. Spiked protein experiments were used to validate serum, plasma (citrate), plasma (EDTA), and plasma (heparin) sample types. Saliva, urine, milk, CSF, and cell and tissue extract samples have not been tested with this kit.
Human Erythropoietin ELISA Kit (EPO) ab274397 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
Save your precious samples
Our 384-well Human Erythropoietin ELISA Kit (EPO) only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- EPO Antibody Pair - BSA and Azide free EPO Antibody Pair - BSA and Azide free ab253419
Related and recommended products
Human Erythropoietin ELISA Kit ab274397 is commonly used to study blood conditions such as anemia and polycythemia vera. Erythropoietin (EPO) plays a major role in the production of red blood cells.
Other ELISA kits to measure blood cell mechanisms and disorders are:
- Human Von Willebrand Factor ELISA kit Human Von Willebrand Factor ELISA Kit ab223864
- Human ICAM1 ELISA kit (CD54) Human ICAM1 ELISA Kit (CD54) ab174445
- Human Leptin ELISA kit Human Leptin ELISA Kit ab179884
Erythropoietin is the principal hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin is a secreted protein produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Human erythropoietin is used for the treatment of anemia. Variations affecting the human EPO gene are associated with microvascular complications of diabetes 2. Human Erythropoietin has 83% amino acids sequence identity to rat Erythropoietin, and 80% identity to the mouse Erythropoietin.
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6 product images
Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)
Example of human Erythropoietin standard curve in Sample Diluent NS.
The Erythropoietin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)
Interpolated concentrations of native Erythropoietin in human serum, plasma and HepG2 cell culture supernatant samples.
The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, Plasma (EDTA) 100%, plasma (heparin) 100% and Hep G2 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 16 mIU/mL in neat serum, 24 mIU/mL in neat plasma (citrate), 20 mIU/mL in neat plasma (EDTA), 20 mIU/mL in neat plasma (heparin) and 66 mIU/mL in neat Hep G2 supernatant.
Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 23.4 mIU/mL with a range of 3.3 – 75.7 mIU/mL.
Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)
Example of human Erythropoietin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)
Interpolated concentration of native Erythropoietin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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