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AB288587

Human ETS1 ELISA Kit

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Human ETS1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ETS1 with a sensitivity of 82.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

EWSR2, ETS1, Protein C-ets-1, p54

3 Images
Sandwich ELISA - Human ETS1 ELISA Kit (AB288587)
  • sELISA

Lab

Sandwich ELISA - Human ETS1 ELISA Kit (AB288587)

Linearity of dilution.

Interpolated concentration of native ETS1 was measured in duplicate at different sample concentrations. Undiluted samples are 250 μg/mL Hela cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human ETS1 ELISA Kit (AB288587)
  • sELISA

Supplier Data

Sandwich ELISA - Human ETS1 ELISA Kit (AB288587)

Representative image of Human ETS1 ELISA Kit (ab288587)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human ETS1 ELISA Kit Capture Antibody

- 10X Human ETS1 Detector Antibody

- 10X Human ETS1 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human ETS1 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human ETS1 ELISA Kit (AB288587)
  • sELISA

Lab

Sandwich ELISA - Human ETS1 ELISA Kit (AB288587)

Example of human ETS1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 82.5 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ETS1 ELISA Kit (ab288587) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ETS1 protein in Cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ETS1 with 10.023 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "90 - 113 %", "average": "= 103" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288587-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288587-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288587-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ETS1 Lyophilized Recombinant Protein", "number":"AB288587-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ETS1 Capture Antibody", "number":"AB288587-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB288587-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288587-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288587-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ETS1 Detector Antibody", "number":"AB288587-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB288587-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288587-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288587-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETS1 also known as E26 Transformation-Specific 1 is a transcription factor protein weighing approximately 51-53 kDa. It belongs to the ETS family of transcription factors and can be referred to by other names such as ETS-1 ETS 1 or Ets-1. This protein shows expression in numerous tissues including the thymus spleen and brain. Importantly ETS1 binds to specific DNA sequences to regulate the transcription of various target genes.
Biological function summary

The function of this protein involves the regulation of gene expression linked to cell development differentiation and apoptosis. ETS1 does not form stable complexes with other proteins in its resting form but it can interact dynamically with co-factors during activation. Its activity plays a role in immune response modulation and vascular development by controlling downstream target genes connected to these processes.

Pathways

The involvement of this protein spans key signal transduction pathways including the MAPK and PI3K/AKT pathways. In these pathways ETS1 regulates gene expression influenced by external stimuli such as growth factors. It interacts with other pathway-related proteins like ERK1/2 and AKT initiating cellular responses that contribute to cell proliferation and survival mechanisms.

Abnormalities in the function or expression of this protein have been linked to certain cancers such as leukemia and breast cancer. The protein interacts with other oncogenic proteins including BRISA and Palikuqi leading to progression of these malignancies. Additionally involvement in autoimmune disorders like lupus has been observed where deregulation of ETS1 can affect immune cell behavior and promote hyperactivation of immune responses.
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Product protocols

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Target data

Transcription factor (PubMed : 10698492, PubMed : 11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed : 20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed : 20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed : 15247905, PubMed : 15592518).. Isoform Ets-1 p27. Acts as a dominant-negative for isoform c-ETS-1A.
See full target information ETS1
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websiteProtocolBooklet
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