AB193759

Human Exodus 2 ELISA Kit (CCL21)

SimpleStep
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(5 Publications)

Human Exodus 2 ELISA Kit (CCL21) is a single-wash 90-min Simplestep used to quantify Human Exodus 2 (CCL21) with a sensitivity of 3.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

SCYA21, UNQ784/PRO1600, CCL21, C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21, SLC

4 Images

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

sELISA

Supplier Data

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

Titration of Human serum within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 3) are graphed

sELISA

Supplier Data

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

Observed Exodus 2 in individual donor normal Human serum (n=10).

Mean Human Exodus 2 is 479 pg/mL and values fall within expected normal reference ranges (WHO).

sELISA

Supplier Data

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

Observed Exodus 2 levels in pooled donor (n=50), mixed gender normal Human plasma and serum.

Mean Human Exodus 2 values fall within expected normal reference ranges (WHO).

sELISA

Supplier Data

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

Example of Exodus 2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.8 pg/mL

Range

9.4 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Exodus 2 ELISA Kit (CCL21) (ab193759) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Exodus 2 (CCL21) protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Exodus 2 (CCL21) with 3.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Exodus 2 (CCL 21) is a small cytokine in the CC chemokine family, and is involved in inhibition of hemopoiesis and stimulation of chemotaxis. It interacts with atypical chemokine receptor ACKR4 and recruits beta-arrestin to ACKR4.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Serum", "range": "102 - 106 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "99 - 106 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "93 - 106 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "94 - 105 %", "average": "= 99" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL21 also known as Exodus 2 or 2H26 is a chemokine with significant roles in the immune system. It is a small protein with a molecular mass of approximately 12 kDa. CCL21 is expressed in high concentrations in secondary lymphoid organs such as the spleen lymph nodes and Peyer's patches. It serves as a chemoattractant particularly for lymphocytes and dendritic cells facilitating their migration towards lymphoid organs.

Biological function summary

CCL21 is essential for guiding immune cells to areas where they are needed for initiating immune responses. It forms a non-covalent complex with its receptor CCR7 a primary mechanism through which it exerts its biological effects. CCL21 coordinates the positioning of immune cells within the lymphoid organs ensuring a functional and effective immune response to pathogens or injuries. Its expression pattern aids in the organization of lymphoid tissue architecture and contributes to efficient trafficking of T-cells and dendritic cells.

Pathways

CCL21 plays an important role in the chemokine signaling pathway and the leukocyte transendothelial migration pathway. Within these pathways CCL21 works closely with its receptor CCR7 to guide immune cell navigation and localization. This collaboration is essential for directing cells to appropriate sites in the immune system aligning CCL21 with similar proteins like CCL19 another chemokine that also interacts with CCR7.

CCL21 is associated with several immune-related conditions including rheumatoid arthritis and chronic lymphocytic leukemia. In rheumatoid arthritis CCL21 contributes to the infiltration and retention of immune cells in the synovial tissue while in chronic lymphocytic leukemia it aids in the homing of malignant cells to lymphoid organs. CCL21’s interaction with CCR7 in these diseases highlights its importance not only in immune surveillance but also in the pathology of these disorders.

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Product protocols

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Target data

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

See full target information CCL21

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 16:4655-4665 PubMed39144301

2024

Differences in T cell-associated serum markers between ischemic cardiomyopathy and dilated cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuli Huang,Lin Xuan,Qiong Xu,Jun Wang,Jie Liu

Open life sciences 18:20220678 PubMed37589000

2023

HP1 induces ferroptosis of renal tubular epithelial cells through NRF2 pathway in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanqiang Zhou,Min Wu,Gaolun Liu,Li Zhou

Bioengineered 12:4004-4015 PubMed34288826

2021

Long non-coding RNA H19 protects against intracerebral hemorrhage injuries via regulating microRNA-106b-5p/acyl-CoA synthetase long chain family member 4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Chen,Haoran Wang,Chenglin Lv,Chongdan Mao,Yuguang Cui

Oncotarget 8:63901-63910 PubMed28969039

2017

EGFR or HER2 inhibition modulates the tumor microenvironment by suppression of PD-L1 and cytokines release.

Applications

ELISA

Species

Unspecified reactive species

Koung Jin Suh,Ji Hea Sung,Jin Won Kim,Song-Hee Han,Hye Seung Lee,Ahrum Min,Mi Hyun Kang,Ji Eun Kim,Ji-Won Kim,Se Hyun Kim,Jeong-Ok Lee,Yu Jung Kim,Keun-Wook Lee,Jee Hyun Kim,Soo-Mee Bang,Seock-Ah Im,Jong Seok Lee

BMJ open respiratory research 2:e000085 PubMed26448865

2015

Lymphangiogenic factors are associated with the severity of hypersensitivity pneumonitis.

Applications

ELISA

Species

Human

Masahiro Yamashita,Takashi Mouri,Miyuki Niisato,Hiroo Nitanai,Hitoshi Kobayashi,Masahito Ogasawara,Ryujin Endo,Kazuki Konishi,Tamotsu Sugai,Takashi Sawai,Kohei Yamauchi

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Exodus 2 ELISA Kit (CCL21)

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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