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AB278116

Human Ezrin ELISA Kit

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Human Ezrin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Ezrin with a sensitivity of 19.37 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

2 Images
Sandwich ELISA - Human Ezrin ELISA Kit (AB278116)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ezrin ELISA Kit (AB278116)

Example of human Ezrin standard curve in Sample Diluent NS

Sandwich ELISA - Human Ezrin ELISA Kit (AB278116)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ezrin ELISA Kit (AB278116)

Interpolated concentrations of native Ezrin in human serum and plasma samples.

The concentrations of Ezrin were measured in duplicates, interpolated from the Ezrin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ezrin concentration was determined to be 3409.74 pg/mL in serum, 1833.59 pg/mL in plasma (citrate), 3172.31 pg/mL in plasma (EDTA), and 3470.33 pg/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 19.37 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Ezrin ELISA kit (ab278116) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Ezrin protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ezrin with 19.37 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Ezrin is a cytoplasmic peripheral protein involved in adhesion, migration, and organization of cytoskeletal structures to the plasma membrane. In epithelial cells, Ezrin is required for the formation of microvilli and membrane ruffles on the apical pole. The N-terminal domain of Ezrin binds to NHERF protein involved in long-range allostery. Along with PLEKHG6, Ezrin is required for normal macropinocytosis. Human Ezrin holds a 97% and 96% sequence homology with mouse and rat Ezrin, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Serum", "range": "84 - 104 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "80 - 100 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "80 - 91 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "80 - 87 %", "average": "= 84" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab278116-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB278116-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB278116-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278116-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278116-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB278116-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB278116-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Ezrin Lyophilized Recombinant Protein", "number":"AB278116-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ezrin Capture Antibody", "number":"AB278116-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB278116-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ezrin Detector Antibody", "number":"AB278116-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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Product protocols

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Target data

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR
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websiteProtocolBooklet
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Product promise

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