Human FABP-1 ELISA Kit

Product details

Human FABP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222875) is designed for the quantitative measurement of human FABP-1 protein in plasma, serum, saliva, milk, cerebral spinal fluid and cell culture samples.

The assay employs a quantitative sandwich enzyme immunoassay technique that measures human FABP-1 in approximately 4 hours. A polyclonal antibody specific for human FABP-1 has been pre-coated onto a 96-well microplate with removable strips. FABP-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human FABP-1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Mitochondrial glutamate oxaloacetate transaminase 2 (FABP-1), also called mitochondrial aspartate aminotransferase (mAspAT) and fatty acid-binding protein, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT is a pyridoxal phosphate-dependent enzyme, which exists in cytoplasmic GOT1 and inner-membrane mitochondrial FABP-1 forms. GOT plays a role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The two enzymes show close homology. FABP-1 is a dimer containing two identical subunits, each 45 kDa with about 401 amino acids. FABP-1 acetylation stimulates the malate–aspartate NADH shuttle activity and oxidative protection. It is also involved in cell proliferation and energy production.