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Human FABP2 ELISA Kit is a Sandwich ELISA kit for the measurement of Human FABP2 in Human in Plasma, Cell culture media, Serum samples.


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Sandwich ELISA - Human FABP2 ELISA Kit (AB193700), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture media, Serum
Assay type
Sandwich
Reactive species
Human
Range
25 - 100000 pg/mL
Sensitivity
= 25 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

Alternative names

FABPI, FABP2, Fatty acid-binding protein 2, Intestinal-type fatty acid-binding protein, I-FABP

What's included?

1 x 96 Tests
Components
1500X HRP-Streptavidin Concentrate
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B
1 x 15 mL
Assay Diluent C
1 x 30 mL
Biotinylated Human FABP2 detection antibody
2 x 1 Vial
Human FABP2 Standards (lyophilized)
2 x 1 Vial
Pre-coated Human FABP2 Microplate (12 strips x 8 wells)
1 x 1 Unit
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human FABP2 ELISA Kit is a Sandwich ELISA kit for the measurement of Human FABP2 in Human in Plasma, Cell culture media, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture media, Serum
Assay type
Sandwich
Reactive species
Human
Range
25 - 100000 pg/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 25 pg/mL

Precision

Intra assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
< 10

Inter assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
< 12

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 129.8
Range
120 - 136 %
Sample type
Plasma
Average %
= 126.5
Range
110 - 135 %
Sample type
Cell culture media
Average %
= 112.4
Range
103 - 123 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's FABP2 Human ELISA Kit (ab193700) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FABP2 in sera, plasma and cell culture supernatants.

This assay employs an antibody specific for human FABP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FABP2 present in the samples. The wells are washed and biotinylated anti-human FABP2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FABP2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human FABP2 ELISA Kit (ab193700), expandable thumbnail

    Sandwich ELISA - Human FABP2 ELISA Kit (ab193700)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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