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AB193700

Human FABP2 ELISA Kit

5

(1 Review)

|

(1 Publication)

Human FABP2 ELISA Kit is a Sandwich ELISA for the measurement of Human FABP2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

FABPI, FABP2, Fatty acid-binding protein 2, Intestinal-type fatty acid-binding protein, I-FABP

1 Images
Sandwich ELISA - Human FABP2 ELISA Kit (AB193700)
  • sELISA

Supplier Data

Sandwich ELISA - Human FABP2 ELISA Kit (AB193700)

Example Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich

Sensitivity

= 25 pg/mL

Range

25 - 100000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's FABP2 Human ELISA Kit (ab193700) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FABP2 in sera, plasma and cell culture supernatants.

This assay employs an antibody specific for human FABP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FABP2 present in the samples. The wells are washed and biotinylated anti-human FABP2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FABP2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "120 - 136 %", "average": "= 129.8" }, { "sample": "Plasma", "range": "110 - 135 %", "average": "= 126.5" }, { "sample": "Cell culture media", "range": "103 - 123 %", "average": "= 112.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193700-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193700-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193700-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FABP2 Standards (lyophilized)", "number":"AB193700-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human FABP2 Microplate (12 strips x 8 wells)", "number":"AB193700-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human FABP2 detection antibody", "number":"AB193700-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB193700-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193700-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193700-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"1500X HRP-Streptavidin Concentrate", "number":"AB193700-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
See full target information FABP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC pediatrics 21:355 PubMed34418984

2021

Serum HMGB1 level is correlated with serum I-FABP level in neonatal patients with necrotizing enterocolitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruyahan Huo,Heng Liu,Jing Chen,Hong Sheng,Li Miao
View all publications
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websiteProtocolBooklet
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