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AB234566

Human FABP2 ELISA Kit

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(1 Publication)

Human FABP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FABP2 with a sensitivity of 1.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

FABPI, FABP2, Fatty acid-binding protein 2, Intestinal-type fatty acid-binding protein, I-FABP

7 Images
ELISA - Human FABP2 ELISA Kit (AB234566)
  • ELISA

Supplier Data

ELISA - Human FABP2 ELISA Kit (AB234566)
Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)
  • sELISA

Supplier Data

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)

Interpolated concentrations of spiked FABP2 in human urine and RPMI media with 10% FBS.

The concentrations of FABP2 were measured in duplicate and interpolated from the FABP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)
  • sELISA

Supplier Data

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)

Example of human FABP2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)
  • sELISA

Supplier Data

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)

Human FABP2 Competitor Std Curve Comparison.

Standard curve comparison between human FABP2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)
  • sELISA

Supplier Data

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration was determined to be 1,094 pg/mL with a range of 422 – 3,639 pg/mL.

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)
  • sELISA

Supplier Data

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)

Interpolated concentrations of native FABP2 in human serum, plasma, and LoVo cell extract samples.

The concentrations of FABP2 were measured in duplicates, interpolated from the FABP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50%, plasma (EDTA) 25%, plasma (citrate) 50%, and LoVo extract 750 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration in the neat samples was determined to be 1,248 pg/mL in serum, 1,104 pg/mL in plasma (heparin), 1,642 pg/mL in plasma (EDTA), and 1,199 pg/mL in plasma (citrate).

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)
  • sELISA

Supplier Data

Sandwich ELISA - Human FABP2 ELISA Kit (AB234566)

Example of human FABP2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.7 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FABP2 ELISA Kit ab234566 is a rapid single-wash 90-min Sandwich ELISA to measure Human FABP2 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 1.7 pg/mL.

How the assay works

Human FABP2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human FABP2 ELISA Kit ab234566 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-I-FABP antibody [EPR21142-172] - BSA and Azide free (Capture) ab245024
- Anti-I-FABP antibody [EPR21142-68] - BSA and Azide free (Detector) ab245024

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" } ]
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Recovery

[ { "sample": "Serum", "range": "111 - 125 %", "average": "= 119" }, { "sample": "EDTA Plasma", "range": "109 - 120 %", "average": "= 115" }, { "sample": "Cell culture extracts", "range": "92 - 97 %", "average": "= 95" }, { "sample": "Urine", "range": "82 - 85 %", "average": "= 83" }, { "sample": "Heparin Plasma", "range": "103 - 110 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "105 - 118 %", "average": "= 113" }, { "sample": "Cell culture media", "range": "98 - 102 %", "average": "= 100" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234566-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234566-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234566-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234566-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234566-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234566-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234566-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FABP2 Lyophilized Recombinant Protein", "number":"AB234566-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB234566-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FABP2 Capture Antibody", "number":"AB234566-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FABP2 Detector Antibody", "number":"AB234566-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234566-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
See full target information FABP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC nutrition 10:12 PubMed38212847

2024

Daily yogurt consumption does not affect bone turnover markers in men and postmenopausal women of Caribbean Latino descent: a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay McGrail,Daniela Vargas-Robles,Mayra Rojas Correa,Lisa C Merrill,Sabrina E Noel,Martha Velez,Ana Maldonado-Contreras,Kelsey M Mangano
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com