AB234565

Human FABP4 ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human FABP4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FABP4 with a sensitivity of 2.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Adipocyte lipid-binding protein, Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, ALBP, A-FABP, AFABP, FABP4

6 Images

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

sELISA

Supplier Data

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Interpolated concentrations of native FABP4 in human THP-1 cell supernatant, THP-1 cell extract, adipocyte cell extract, and heart extract.

The concentrations of FABP4 were measured in duplicate and interpolated from the FABP4 standard curve and corrected for sample dilution. Undiluted samples are as follows : THP-1 cell supernatant 50%, THP-1 cell extract 50 μg/mL, adipocyte cell extract 10 μg/mL, and heart extract 1,000 ng/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Serum from ten individual healthy human female and male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP4 concentration was determined to be 18 ng/mL with a range of 7 – 33 ng/mL in the female donors and 12 ng/mL with a range of 8 – 15 ng/mL in the male donors.

sELISA

Supplier Data

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Interpolated concentrations of native FABP4 in human serum and plasma samples.

The concentrations of FABP4 were measured in duplicates, interpolated from the FABP4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 80, plasma (heparin) 1 : 67, plasma (EDTA) 1 : 67, and plasma (citrate) 1 : 67. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP4 concentration in the neat samples was determined to be 13 ng/mL in serum, 12 ng/mL in plasma (heparin), 7 ng/mL in plasma (EDTA), and 11 ng/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Example of human FABP4 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Example of human FABP4 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Human FABP4 Competitor Std Curve Comparison.

Standard curve comparison between human FABP4 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.7 pg/mL

Range

4.69 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human FABP4 ELISA Kit (ab234565) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP4 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP4 with 2.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "105 - 111 %", "average": "= 107" }, { "sample": "Serum", "range": "101 - 107 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "104 - 106 %", "average": "= 105" }, { "sample": "Cell culture extracts", "range": "97 - 108 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "98 - 108 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "104 - 108 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "107 - 110 %", "average": "= 108" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fatty acid-binding protein 4 (FABP4) also known as aP2 is a member of the fatty acid-binding protein family. It predominantly exists in adipocytes and macrophages serving an important role in lipid metabolism and transport. FABP4 facilitates the binding and transport of long-chain fatty acids and other hydrophobic molecules within cells. The molecular weight of FABP4 is approximately 14-15 kDa. It exhibits high expression levels in adipose tissue and is inducible in macrophages under certain conditions.

Biological function summary

This protein participates in the regulation of lipid homeostasis and energy metabolism. FABP4 is part of the lipid-binding protein family that helps maintain fatty acid equilibrium within cells. By controlling the transport and signaling of lipids FABP4 impacts lipid storage insulin sensitivity and inflammation processes. Its activity is not confined to a specific multimeric complex yet it interacts with other proteins to exert its functions contributing to significant metabolic pathways in the body.

Pathways

FABP4 plays essential roles in the regulation of metabolic and inflammatory signaling pathways. It is intricately involved in the peroxisome proliferator-activated receptor gamma (PPARγ) pathway where it modulates adipose tissue development and glucose metabolism. In addition FABP4 has connections with the c-Jun N-terminal kinase (JNK) signaling pathway influencing stress and inflammatory responses. Interactions with these pathways place FABP4 at a link with proteins like PPARγ and JNK essential for cellular metabolic balance and responses to metabolic stress.

Researchers connect FABP4 to conditions such as obesity and type 2 diabetes. Its expression levels correlate with insulin resistance and metabolic syndrome making it a target of interest in these metabolic disorders. FABP4 interacts with other proteins like adiponectin which is important in modulating glucose levels and fatty acid breakdown. Understanding FABP4's role in these pathways can facilitate the development of therapeutic strategies aimed at targeting metabolic dysfunctions associated with obesity and diabetes.

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Product protocols

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Target data

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

See full target information FABP4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 16: PubMed38139788

2023

The Influence of Nucleoside Reverse Transcriptase Inhibitors on Mitochondrial Activity, Lipid Content, and Fatty-Acid-Binding Protein Levels in Microglial HMC3 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Lipke,Adriana Kubis-Kubiak,Agnieszka Piwowar

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human FABP4 ELISA Kit

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Sandwich ELISA - Human FABP4 ELISA Kit (AB234565)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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