Human FABP5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FABP5 with a sensitivity of 0.14 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Tissue Extracts, Heparin Plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.31 - 20 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.14 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, that regulate the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors (PubMed:21395585, PubMed:22170058). Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta; which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation (By similarity). May be involved in keratinocyte differentiation (PubMed:8092987).
Alternative names
Fatty acid-binding protein 5, Epidermal-type fatty acid-binding protein, Psoriasis-associated fatty acid-binding protein homolog, E-FABP, PA-FABP, FABP5
What's included?
Recommended products
Human FABP5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FABP5 with a sensitivity of 0.14 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Tissue Extracts, Heparin Plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.31 - 20 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.14 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 105 | Range 94 - 116 % |
Sample type EDTA Plasma | Average % = 83 | Range 81 - 84 % |
Sample type Saliva | Average % = 99 | Range 96 - 102 % |
Sample type Urine | Average % = 83 | Range 81 - 83 % |
Sample type Tissue Extracts | Average % = 92 | Range 87 - 100 % |
Sample type Heparin Plasma | Average % = 93 | Range 81 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human FABP5 ELISA kit (ab272474) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human FABP5 protein in serum, plasma, urine, saliva and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP5 with 0.14 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Fatty acid-binding protein 5 (FABP5), also known as E-FABP for Epidermal-type fatty acid-binding protein, is a member of the fatty acid binding proteins and is encoded by the FABP5 gene. FABP5 is an a 134- amino acid containing protein that can be found either as a monomer or a homodimer. FABP5 mainly serves as an intracellular carrier for long-chain fatty acids and related lipids, which regulates metabolism. FABP5 can also transport specific fatty acids, such as retinoic acid and endocannabinoid, from the cytosol to the nucleus, where they activate nuclear receptors.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FABP5 protein also known as Fatty Acid Binding Protein 5 or Epidermal-type Fatty Acid Binding Protein (E-FABP) serves an important role in the transport and metabolism of long-chain fatty acids. It has a molecular mass of approximately 15 kDa. FABP5 is expressed in multiple tissues with notably high expression in the epidermis brain liver and adipose tissue. This protein assists in intracellular fatty acid movement affecting cellular lipid catabolism.
- Biological function summary
FABP5 interacts with specific ligands such as long-chain fatty acids which influences cellular processes like growth differentiation and energy homeostasis. It is not known to be part of a larger complex. This protein is integral in regulating lipid signaling pathways and responses due to its binding capacity which modulates the availability of fatty acids for metabolic processes. Its expression pattern hints at a possible role in the body's adaptation to dietary lipid intake.
- Pathways
FABP5 holds a significant position in the peroxisome proliferator-activated receptor (PPAR) signaling pathway and the fatty acid metabolism pathway. In the PPAR signaling pathway FABP5 influences the activity of PPARβ/δ receptors which regulate gene expression involved in lipid metabolism and energy homeostasis. It is related to other fatty acid binding proteins such as FABP4 which similarly participate in lipid metabolic processes and have roles in the regulation of lipid and glucose metabolism.
- Associated diseases and disorders
Researchers have linked FABP5 to conditions like psoriasis and type 2 diabetes. In psoriasis the overexpression of FABP5 has been observed in skin lesions suggesting that it might contribute to the pathological process. FABP5 is also connected to metabolic syndrome and insulin resistance as its dysregulation can influence lipid and glucose metabolism processes. In these disorders FABP5 interacts with proteins like PPARγ indicating possible intervention points for therapeutic targeting.
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4 product images
Sandwich ELISA - Human FABP5 ELISA Kit (ab272474)
Example of human FABP5 standard curve in Sample Diluent NS.
The FABP5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human FABP5 ELISA Kit (ab272474)
Interpolated concentrations of native FABP5 in human saliva.
The concentrations of FABP5 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 1.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 766 ng/mL in saliva.
Sandwich ELISA - Human FABP5 ELISA Kit (ab272474)
Interpolated concentrations of native FABP5 in human urine.
The concentrations of FABP5 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 8.2 ng/mL in urine.
Sandwich ELISA - Human FABP5 ELISA Kit (ab272474)
Serum from eight individual healthy FABP5 male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP5 concentration was determined to be 3.4 ng/mL with a range of 0.8 – 11.2 ng/mL.
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