Human FABP5 ELISA Kit
- Recombinant
- SimpleStep
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Human FABP5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FABP5 with a sensitivity of 0.14 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Fatty acid-binding protein 5, Epidermal-type fatty acid-binding protein, Psoriasis-associated fatty acid-binding protein homolog, E-FABP, PA-FABP, FABP5
- sELISA
Supplier Data
Sandwich ELISA - Human FABP5 ELISA Kit (AB272474)
Serum from eight individual healthy FABP5 male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP5 concentration was determined to be 3.4 ng/mL with a range of 0.8 – 11.2 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human FABP5 ELISA Kit (AB272474)
Interpolated concentrations of native FABP5 in human saliva.
The concentrations of FABP5 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 1.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 766 ng/mL in saliva.
- sELISA
Supplier Data
Sandwich ELISA - Human FABP5 ELISA Kit (AB272474)
Interpolated concentrations of native FABP5 in human urine.
The concentrations of FABP5 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 8.2 ng/mL in urine.
- sELISA
Supplier Data
Sandwich ELISA - Human FABP5 ELISA Kit (AB272474)
Example of human FABP5 standard curve in Sample Diluent NS.
The FABP5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human FABP5 ELISA kit (ab272474) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human FABP5 protein in serum, plasma, urine, saliva and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP5 with 0.14 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Fatty acid-binding protein 5 (FABP5), also known as E-FABP for Epidermal-type fatty acid-binding protein, is a member of the fatty acid binding proteins and is encoded by the FABP5 gene. FABP5 is an a 134- amino acid containing protein that can be found either as a monomer or a homodimer. FABP5 mainly serves as an intracellular carrier for long-chain fatty acids and related lipids, which regulates metabolism. FABP5 can also transport specific fatty acids, such as retinoic acid and endocannabinoid, from the cytosol to the nucleus, where they activate nuclear receptors.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FABP5 interacts with specific ligands such as long-chain fatty acids which influences cellular processes like growth differentiation and energy homeostasis. It is not known to be part of a larger complex. This protein is integral in regulating lipid signaling pathways and responses due to its binding capacity which modulates the availability of fatty acids for metabolic processes. Its expression pattern hints at a possible role in the body's adaptation to dietary lipid intake.
Pathways
FABP5 holds a significant position in the peroxisome proliferator-activated receptor (PPAR) signaling pathway and the fatty acid metabolism pathway. In the PPAR signaling pathway FABP5 influences the activity of PPARβ/δ receptors which regulate gene expression involved in lipid metabolism and energy homeostasis. It is related to other fatty acid binding proteins such as FABP4 which similarly participate in lipid metabolic processes and have roles in the regulation of lipid and glucose metabolism.
Product protocols
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