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AB300318

Human Factor B ELISA Kit

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Human Factor B ELISA Kit is a sandwich ELISA designed to quantify Human Factor B with a sensitivity of 18.18 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 53.125 - 3400 pg/mL

View Alternative Names

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG

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ELISA - Human Factor B ELISA Kit (AB300318)
  • ELISA

Supplier Data

ELISA - Human Factor B ELISA Kit (AB300318)
Sandwich ELISA - Human Factor B ELISA Kit (AB300318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)

Interpolated concentrations of Factor B in normal human serum donors.

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor B concentration was determined to be 255 μg/mL with a range of 178 – 331 μg/mL.

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)

Example of human Factor B standard curve in Sample Diluent NS.

Example of human Factor B standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)

Interpolated concentrations of human Factor B in HepG2 cell culture supernatant, saliva, and milk.

Interpolated concentration of native Factor B was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell culture supernatant 1 : 8, saliva 1 : 16, and milk 1 : 800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)

Interpolated concentrations of human Factor B in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native Factor B was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 18.18 pg/mL

Range

53.125 - 3400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Factor B ELISA Kit ab300318 is a rapid single-wash 90-min Sandwich ELISA to measure Human Factor B in cell culture supernatant, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 18.18 pg/mL.

How the assay works

Human Factor B SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Factor B ELISA Kit ab300318 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Factor B antibody [EPR24245-109] - BSA and Azide free (Capture) ab302723
- Anti-Factor B antibody [EPR24287-95] - BSA and Azide free (Detector) ab302723
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.72" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 100 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "103 - 110 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "92 - 106 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Serum", "range": "88 - 94 %", "average": "= 91" } ]
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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab300318-96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB300318-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300318-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB300318-CMP03", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300318-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Factor B Capture Antibody", "number":"AB300318-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB300318-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Factor B Lyophilized Recombinant Protein", "number":"AB300318-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Factor B Detector Antibody", "number":"AB300318-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300318-CMP06", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB300318-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.
Biological function summary

Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.

Pathways

Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.

Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.
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Product protocols

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Target data

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments : Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
See full target information CFB
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