Human Factor B ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Factor B in Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 53.125 - 3400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 18.18 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Alternative names
BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG
What's included?
Recommended products
Human Factor B ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Factor B in Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 53.125 - 3400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 18.18 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5.72 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 91 | Range 86 - 100 % |
Sample type Citrate plasma | Average % = 105 | Range 103 - 110 % |
Sample type EDTA Plasma | Average % = 99 | Range 92 - 106 % |
Sample type Heparin Plasma | Average % = 96 | Range 94 - 98 % |
Sample type Serum | Average % = 91 | Range 88 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.
- Biological function summary
Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.
- Pathways
Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.
- Associated diseases and disorders
Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.
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4 product images
Sandwich ELISA - Human Factor B ELISA Kit (ab300318)
Interpolated concentrations of Factor B in normal human serum donors.
Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor B concentration was determined to be 255 μg/mL with a range of 178 – 331 μg/mL.
Sandwich ELISA - Human Factor B ELISA Kit (ab300318)
Example of human Factor B standard curve in Sample Diluent NS.
Example of human Factor B standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Factor B ELISA Kit (ab300318)
Interpolated concentrations of human Factor B in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).
Interpolated concentration of native Factor B was measured in duplicate at different sample concentrations. Undiluted samples are 1:100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Factor B ELISA Kit (ab300318)
Interpolated concentrations of human Factor B in HepG2 cell culture supernatant, saliva, and milk.
Interpolated concentration of native Factor B was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HepG2 cell culture supernatant 1:8, saliva 1:16, and milk 1:800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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