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AB99969

Human Factor D ELISA Kit

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(4 Publications)

Human Factor D ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Factor D in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

DF, PFD, CFD, Complement factor D, Adipsin, C3 convertase activator, Properdin factor D

2 Images
Sandwich ELISA - Human Factor D ELISA Kit (AB99969)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor D ELISA Kit (AB99969)

Representative Standard Curve using ab99969.

Sandwich ELISA - Human Factor D ELISA Kit (AB99969)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor D ELISA Kit (AB99969)

Representative Standard Curve using ab99969.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 4 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human Factor D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Factor D in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Factor D coated on a 96-well plate. Standards and samples are pipetted into the wells and Factor D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Factor D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Factor D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "115 - 133 %", "average": "= 126.4" }, { "sample": "Serum", "range": "118 - 139 %", "average": "= 130.3" }, { "sample": "Plasma", "range": "107 - 140 %", "average": "= 131.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99969-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99969-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99969-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-human Adipsin", "number":"AB99969-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99969-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99969-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant human Fatcor D Standards (lyophilized)", "number":"AB99969-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99969-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB99969-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Factor D Microplate (12 strips x 8 wells)", "number":"AB99969-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor D also known as complement factor D or CFD protein is a serine protease involved in the alternative pathway of the complement system. It plays a mechanical role by cleaving factor B when it is bound to C3b forming the active C3 convertase C3bBb. This enzyme is relatively small with a mass of approximately 28.5 kDa. It is mainly expressed in adipose tissue but it can also be found in the blood plasma.
Biological function summary

Factor D aids the immune system's ability to destroy pathogens. As part of the alternative complement pathway it acts independently and does not associate with larger protein complexes. Its activation of the alternative pathway leads to direct lysis of pathogens therefore contributing to innate immunity.

Pathways

Factor D participates specifically in the complement activation pathway particularly in the innate immune response. It works closely with other proteins in the complement system such as factor B and C3. This pathway enhances the opsonization and removal of microbes and damaged cells acting in parallel with the classical pathway of the complement system.

Abnormal function or regulation of factor D can relate to chronic inflammation and age-related macular degeneration. Disorders involving factor D may involve excess complement activation leading to tissue damage. The role of factor D in these diseases often connects it to other key complement components such as C3 and factor B which can also become problematic when dysregulated.
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Product protocols

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Target data

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
See full target information CFD
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Pakistan journal of medical sciences 36:1495-1499 PubMed33235563

2020

Impact of Gastric Sleeve Surgery on Plasma Retinol Binding Protein 4 and Adipsin Levels in Healthy Male Population.

Applications

Unspecified application

Species

Unspecified reactive species

Suad Alshubrami,Khalid Al-Regaiey,Assim A Alfadda,Muhammad Iqbal

The Journal of clinical endocrinology and metabolism 104:5703-5714 PubMed31390002

2019

Effects of Nicotinamide Riboside on Endocrine Pancreatic Function and Incretin Hormones in Nondiabetic Men With Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Ole L Dollerup,Samuel A J Trammell,Bolette Hartmann,Jens J Holst,Britt Christensen,Niels Møller,Matthew P Gillum,Jonas T Treebak,Niels Jessen

Scientific reports 6:20073 PubMed26832661

2016

Serum Adipsin Levels throughout Normal Pregnancy and Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia E Poveda,María F Garcés,Carlos E Ruiz-Linares,Diana Varón,Sergio Valderrama,Elizabeth Sanchez,Adriana Castiblanco-Cortes,Yessica Agudelo-Zapata,Héctor Fabio Sandoval-Alzate,Luis G Leal,Edith Ángel-Müller,Ariel I Ruíz-Parra,Angélica M González-Clavijo,Carlos Diéguez,Rubén Nogueiras,Jorge E Caminos

Science translational medicine 7:297ra116 PubMed26203084

2015

Inhibition of the alternative complement pathway preserves photoreceptors after retinal injury.

Applications

Unspecified application

Species

Unspecified reactive species

J Harry Sweigard,Hidetaka Matsumoto,Kaylee E Smith,Leo A Kim,Eleftherios I Paschalis,Yoko Okonuki,Alexandra Castillejos,Keiko Kataoka,Eiichi Hasegawa,Ryoji Yanai,Deeba Husain,John D Lambris,Demetrios Vavvas,Joan W Miller,Kip M Connor
View all publications
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