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AB300307

Human Factor IX/PTC ELISA Kit

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Human Factor IX/PTC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor IX/PTC with a sensitivity of 15.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9

9 Images
Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Example of Human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 400 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Example of human Factor IX/PTC standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Example of Human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Standard curve comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 15.884 pg/mL

Range

97.656 - 6250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Factor IX/PTC ELISA Kit ab300307 is a rapid single-wash 90-min ELISA kit to measure Human Factor IX/PTC in serum and plasma. This SimpleStep ELISA® sensitivity is 15.884 pg/mL.

How the assay works
Human Factor IX/PTC SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit is designed for the quantification of human Factor IX/PTC. The standard protein in this kit is full length human Factor IX/PTC. Native signal was detected in serum, plasma (heparin), plasma (EDTA), and plasma (citrate). Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.

Human Factor IX/PTC ELISA Kit ab300307 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

Save your precious samples
Our 384-well Human Factor IX/PTC ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Factor IX (F9) Antibody Pair - BSA and Azide free ab253721
- Anti-Factor IX/PTC antibody [EPR22846-57] - BSA and Azide free (Capture) ab281281
- Anti-Factor IX/PTC antibody [EPR22846-233] - BSA and Azide free (Detector) ab281131

Related and recommended products
Human Factor IX/PTC ELISA Kit ab300307 is commonly used to study cardiovascular diseases and clotting disorders.

Other ELISA kits to measure inflammation and coagulation include:
- Human Factor X ELISA Kit ab108832
- Human Lipocalin-2 ELISA Kit ab215541

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]
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Recovery

[ { "sample": "Citrate plasma", "range": "111 - 119 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "95 - 115 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "103 - 118 %", "average": "= 112" }, { "sample": "Serum", "range": "92 - 113 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
See full target information Coagulation factor IX
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websiteProtocolBooklet
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