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Human Factor IX/PTC ELISA Kit ab300307 is a single-wash 90-min SimpleStep ELISA used to quantify Human Factor IX/PTC with a sensitivity of 15.884 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Human Factor IX/PTC sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation


Images

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307), expandable thumbnail
  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307), expandable thumbnail
  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307), expandable thumbnail
  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307), expandable thumbnail
  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

97.656 - 6250 pg/mL

Assay time

1h 30m

Sensitivity

= 15.884 pg/mL

Abcam Recommends

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

Alternative names

What's included?

1 x 384 Tests
Components
10X Human Factor IX/PTC Capture Antibody
1 x 600 µL
10X Human Factor IX/PTC Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
Antibody Diluent 4BI
1 x 6 mL
Human Factor IX/PTC Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seal
1 x 1 Unit
Sample Diluent NS (ab193972)
2 x 50 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

Human Factor IX/PTC ELISA Kit ab300307 is a single-wash 90-min SimpleStep ELISA used to quantify Human Factor IX/PTC with a sensitivity of 15.884 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Human Factor IX/PTC sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

97.656 - 6250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 15.884 pg/mL

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

7.4

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

10.1

Recovery

Sample specific recovery

Sample type

Citrate plasma

Average %

= 116

Range

111 - 119 %

Sample type

EDTA Plasma

Average %

= 106

Range

95 - 115 %

Sample type

Heparin Plasma

Average %

= 112

Range

103 - 118 %

Sample type

Serum

Average %

= 105

Range

92 - 113 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Factor IX/PTC SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor IX/PTC protein in human serum and plasma. Quantitate Human Factor IX/PTC with 15.884 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Factor IX also known as Christmas factor or PTC is an important clotting protein in the coagulation cascade. It is a 415 amino acid protein with a mass of approximately 55 kDa. Factor IX is mainly expressed in the liver and then released into the bloodstream where it plays a significant role in blood clotting processes. The recombinant version of this protein referred to as factor IX recombinant is used therapeutically especially in patients who require coagulation support due to factor deficiencies.

Biological function summary

The function of factor IX involves its role in the amplification phase of the coagulation cascade. It participates as part of the intrinsic tenase complex which forms on negatively charged phospholipids in the presence of calcium ions. This complex significantly enhances the conversion of factor X to its active form factor Xa an important step in the clotting process. The activity of factor IX can be assessed using assays such as the factor IX activity assay and ELISA allowing for precise measurement of its function and quantity.

Pathways

Factor IX is integral within the intrinsic pathway of blood coagulation. It interacts with proteins such as factor VIII and factor X. When activated factor IXa forms a complex with the cofactor factor VIIIa on membrane surfaces intensifying the transformation of factor X to factor Xa which then catalyzes the conversion of prothrombin to thrombin. This series of reactions leads to fibrin formation important for stable clot production. Factor IXa also relates to the pathways influencing cellular signaling and hemostasis.

Associated diseases and disorders

Factor IX deficiencies cause hemophilia B a genetic disorder that leads to improper blood clotting. This disorder is mainly characterized by spontaneous bleeding or severe bleeding after injury. Patients with hemophilia B usually have mutations in the factor IX gene on the X chromosome affecting the protein's function. Successful treatment often involves replacement therapy using factor IX concentrates or the recombinant form. The interaction with proteases such as factor VIIa highlights its importance in therapeutic strategies for bleeding disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)

    Standard curve comparison between the original Human Factor IX/PTC SimpleStep ELISA (Human Factor IX ELISA Kit ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)

    Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (Human Factor IX ELISA Kit ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)

    Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)

    Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)

    Example of Human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)

    Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:400 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)

    Example of human Factor IX/PTC standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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