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AB325625

Human Factor IX/PTC ELISA Kit, Chemiluminescent

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Human Factor IX/PTC ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Factor IX/PTC with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 13.72 - 30000 pg/ml with a sensitivity of 5.9 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending

View Alternative Names

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9

5 Images
Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)

Standard curve comparison between the Chemiluminescent Human Factor IX SimpleStep ELISA and colorimetric Human Factor IX SimpleStep ELISA (ab300307) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)

Dynamic range comparison between the Chemiluminescent Human Factor IX SimpleStep ELISA, colorimetric Human Factor IX SimpleStep ELISA (ab300307), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Chemiluminescent (AB325625)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867 ng/mL with a range of 1,062 – 8,981 ng/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.9 pg/mL

Range

13.72 - 30000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Factor IX/PTC ELISA Kit, Chemiluminescent (ab325625) is a rapid single-wash sandwich 55 min ELISA to measure Human Factor IX/PTC in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma with a sensitivity of 5.9 pg/mL.

How the assay works

Human Factor IX/PTC ELISA Kit, Chemiluminescent (ab325625) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Factor IX/PTC (ab325625) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Factor IX/PTC antibody [EPR22846-57] - BSA and Azide free (Capture) ab281281
- Anti-Factor IX/PTC antibody [EPR22846-233] - BSA and Azide free (Detector) ab281131
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.1" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 113 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "111 - 119 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "95 - 115 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "103 - 118 %", "average": "= 112" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa (PubMed : 8295821, PubMed : 2592373, PubMed : 20121197, PubMed : 20121198, PubMed : 1730085, PubMed : 19846852, PubMed : 39880037).
See full target information Coagulation factor IX
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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