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Human Factor IX/PTC ELISA Kit, Fluorescent is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Factor IX/PTC , Fluorescent in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

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Images

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325), expandable thumbnail
  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325), expandable thumbnail
  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325), expandable thumbnail
  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325), expandable thumbnail

Key facts

Detection method

Fluorescent

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

48.8 - 25000 pg/mL

Assay time

1h 30m

Sensitivity

= 25.76 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

Alternative names

What's included?

96 Tests
Components
10X Human Factor IX/PTC, Fluorescent Capture Antibody
1 x 600 µL
10X Human Factor IX/PTC, Fluorescent Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Human Factor IX/PTC, Fluorescent Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Factor IX/PTC ELISA Kit, Fluorescent is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Factor IX/PTC , Fluorescent in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

Key facts

Detection method

Fluorescent

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

48.8 - 25000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 25.76 pg/mL

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

7.4

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

10.1

Recovery

Sample specific recovery

Sample type

Citrate plasma

Average %

= 116

Range

111 - 119 %

Sample type

EDTA Plasma

Average %

= 106

Range

95 - 115 %

Sample type

Heparin Plasma

Average %

= 112

Range

103 - 118 %

Sample type

Serum

Average %

= 105

Range

92 - 113 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Factor IX also known as Christmas factor or PTC is an important clotting protein in the coagulation cascade. It is a 415 amino acid protein with a mass of approximately 55 kDa. Factor IX is mainly expressed in the liver and then released into the bloodstream where it plays a significant role in blood clotting processes. The recombinant version of this protein referred to as factor IX recombinant is used therapeutically especially in patients who require coagulation support due to factor deficiencies.

Biological function summary

The function of factor IX involves its role in the amplification phase of the coagulation cascade. It participates as part of the intrinsic tenase complex which forms on negatively charged phospholipids in the presence of calcium ions. This complex significantly enhances the conversion of factor X to its active form factor Xa an important step in the clotting process. The activity of factor IX can be assessed using assays such as the factor IX activity assay and ELISA allowing for precise measurement of its function and quantity.

Pathways

Factor IX is integral within the intrinsic pathway of blood coagulation. It interacts with proteins such as factor VIII and factor X. When activated factor IXa forms a complex with the cofactor factor VIIIa on membrane surfaces intensifying the transformation of factor X to factor Xa which then catalyzes the conversion of prothrombin to thrombin. This series of reactions leads to fibrin formation important for stable clot production. Factor IXa also relates to the pathways influencing cellular signaling and hemostasis.

Associated diseases and disorders

Factor IX deficiencies cause hemophilia B a genetic disorder that leads to improper blood clotting. This disorder is mainly characterized by spontaneous bleeding or severe bleeding after injury. Patients with hemophilia B usually have mutations in the factor IX gene on the X chromosome affecting the protein's function. Successful treatment often involves replacement therapy using factor IX concentrates or the recombinant form. The interaction with proteases such as factor VIIa highlights its importance in therapeutic strategies for bleeding disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325)

    Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325)

    Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (Human Factor IX ELISA Kit ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300325).

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325)

    Example of human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325), expandable thumbnail

    Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325)

    Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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