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AB300325

Human Factor IX/PTC ELISA Kit, Fluorescent

  • Recombinant
  • SimpleStep
  • CatchPoint SimpleStep ELISA
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Human Factor IX/PTC ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Factor IX/PTC with a sensitivity of 25.76 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9

7 Images
Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)

Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300325).

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)

Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300325).

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)

Example of human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit, Fluorescent (AB300325)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.

Key facts

Detection method

Fluorescent

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 25.76 pg/mL

Range

48.8 - 25000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]
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Recovery

[ { "sample": "Citrate plasma", "range": "111 - 119 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "95 - 115 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "103 - 118 %", "average": "= 112" }, { "sample": "Serum", "range": "92 - 113 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor IX also known as Christmas factor or PTC is an important clotting protein in the coagulation cascade. It is a 415 amino acid protein with a mass of approximately 55 kDa. Factor IX is mainly expressed in the liver and then released into the bloodstream where it plays a significant role in blood clotting processes. The recombinant version of this protein referred to as factor IX recombinant is used therapeutically especially in patients who require coagulation support due to factor deficiencies.
Biological function summary

The function of factor IX involves its role in the amplification phase of the coagulation cascade. It participates as part of the intrinsic tenase complex which forms on negatively charged phospholipids in the presence of calcium ions. This complex significantly enhances the conversion of factor X to its active form factor Xa an important step in the clotting process. The activity of factor IX can be assessed using assays such as the factor IX activity assay and ELISA allowing for precise measurement of its function and quantity.

Pathways

Factor IX is integral within the intrinsic pathway of blood coagulation. It interacts with proteins such as factor VIII and factor X. When activated factor IXa forms a complex with the cofactor factor VIIIa on membrane surfaces intensifying the transformation of factor X to factor Xa which then catalyzes the conversion of prothrombin to thrombin. This series of reactions leads to fibrin formation important for stable clot production. Factor IXa also relates to the pathways influencing cellular signaling and hemostasis.

Factor IX deficiencies cause hemophilia B a genetic disorder that leads to improper blood clotting. This disorder is mainly characterized by spontaneous bleeding or severe bleeding after injury. Patients with hemophilia B usually have mutations in the factor IX gene on the X chromosome affecting the protein's function. Successful treatment often involves replacement therapy using factor IX concentrates or the recombinant form. The interaction with proteases such as factor VIIa highlights its importance in therapeutic strategies for bleeding disorders.
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Product protocols

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Target data

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
See full target information Coagulation factor IX
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websiteProtocolBooklet
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