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Human Factor IX/PTC ELISA Kit, Fluorescent is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Factor IX/PTC , Fluorescent in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

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Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

48.8 - 25000 pg/mL

Assay time

1h 30m

Sensitivity

= 25.76 pg/mL

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Shipping To:
United States

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

96 Tests
Components
10X Human Factor IX/PTC, Fluorescent Capture Antibody
1 x 600 µL
10X Human Factor IX/PTC, Fluorescent Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Human Factor IX/PTC, Fluorescent Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Human Factor IX/PTC ELISA Kit, Fluorescent is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Factor IX/PTC , Fluorescent in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

48.8 - 25000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 25.76 pg/mL

Precision

Intra assay

Sample
Serum
n
8
mean
-
SD
-
C.V.
7.4

Inter assay

Sample
Serum
n
3
mean
-
SD
-
C.V.
10.1

Recovery

Sample specific recovery

Sample type
Citrate plasma
Average %
= 116
Range
111 - 119 %
Sample type
EDTA Plasma
Average %
= 106
Range
95 - 115 %
Sample type
Heparin Plasma
Average %
= 112
Range
103 - 118 %
Sample type
Serum
Average %
= 105
Range
92 - 113 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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