Human Factor V ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Factor V in Human in Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Urine samples.
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- iScience 25:1039712022Coagulation factor V is a T-cell inhibitor expressed by leukocytes in COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wang,Prasanti Kotagiri,Paul A Lyons,Rafia S Al-Lamki,Federica Mescia,Laura Bergamaschi,Lorinda Turner,Michael D Morgan,Fernando J Calero-Nieto,Karsten Bach,Nicole Mende,Nicola K Wilson,Emily R Watts,Patrick H Maxwell,Patrick F Chinnery,Nathalie Kingston,Sofia Papadia,Kathleen E Stirrups,Neil Walker,Ravindra K Gupta,David K Menon,Kieren Allinson,Sarah J Aitken,Mark Toshner,Michael P Weekes,James A Nathan,Sarah R Walmsley,Willem H Ouwehand,Mary Kasanicki,Berthold Göttgens,John C Marioni,Kenneth G C Smith,Jordan S Pober,John R BradleyPubMed 35224470
- Thrombosis research 200:91-982021Procoagulant activity in high grade serous ovarian cancer patients following neoadjuvant chemotherapy-The role of the activated protein C pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark P Ward,Feras Abu Saadeh,Sharon A O'Toole,John J O'Leary,Noreen Gleeson,Lucy A NorrisPubMed 33571724
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.5 - 10 ng/mL
- Assay time
- 4h
- Sensitivity
- > 49 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
Alternative names
Coagulation factor V, Activated protein C cofactor, F5
What's included?
Recommended products
Human Factor V ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Factor V in Human in Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.5 - 10 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- > 49 pg/mL
- General recovery
- 102%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Milk | Average % | Range 91 - 103 % |
Sample type Plasma | Average % | Range 94 - 104 % |
Sample type Urine | Average % | Range 94 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Factor V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor V in plasma, urine, milk, and cell culture supernatants.
A Factor V specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor V specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor V captured in plate.
Factor V Standard is a native protein.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Factor V also known as proaccelerin is a coagulation factor playing a main role in blood clotting. This protein with a molecular mass of approximately 330 kDa is synthesized in the liver and found in plasma. The Factor V protein is essential for the coagulation cascade functioning as a pivotal mediator in the conversion of prothrombin to thrombin. It exists in plasma in an inactive form until activated by thrombin or factor Xa to fulfill its mechanical function in hemostasis.
- Biological function summary
The mechanism of Factor V involves its function as a cofactor to Factor Xa in the prothrombinase complex. This complex formation enhances the efficiency of thrombin generation considerably accelerating clot formation. Factor V once converted to its active form aids in forming a stable clot by bridging and immobilizing cellular elements and fibrin strands. Expression occurs mainly in the liver but it also manifests in platelets where it enhances thrombin production during platelet activation and aggregation.
- Pathways
The contribution of Factor V to coagulation is central within the hemostasis pathway specifically the intrinsic and extrinsic pathways of the coagulation cascade. Factor V binds with Factor X to form the prothrombinase complex which is important for the conversion of prothrombin to thrombin. Other proteins such as Factor VIII interact within this pathway to ensure blood clots form properly. Any disturbances in Factor V activity can significantly alter the normal flow of these pathways affecting thrombin generation and subsequent clotting.
- Associated diseases and disorders
Factor V mutations or deficiencies associate with conditions like Factor V Leiden and Factor V deficiency. Factor V Leiden is a genetic mutation that elevates the risk of thrombophilia leading to increased clot formation in veins. On the other hand a deficiency in Factor V can lead to Parahemophilia characterized by a tendency for excessive bleeding. In both conditions the abnormal activity of Factor V affects the interaction with proteins in the coagulation cascade especially impacting the prothrombinase complex and Factor X.
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1 product image
Sandwich ELISA - Human Factor V ELISA Kit (ab137976)
Representative Standard Curve using ab137976
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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