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AB137976

Human Factor V ELISA Kit

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(2 Publications)

Human Factor V ELISA Kit is a Sandwich ELISA for the measurement of Human Factor V in Human in Biofluids samples.

View Alternative Names

Coagulation factor V, Activated protein C cofactor, F5

2 Images
Sandwich ELISA - Human Factor V ELISA Kit (AB137976)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB137976)

Representative Standard Curve using ab137976

Sandwich ELISA - Human Factor V ELISA Kit (AB137976)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB137976)

Representative Standard Curve using ab137976

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Milk, Plasma, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

> 54 pg/mL

Range

2.5 - 10 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Factor V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor V in plasma, urine, milk, and cerebrospinal fluid.

A Factor V specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor V specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor V captured in plate.

Factor V Standard is a native protein.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Milk", "range": "91 - 103 %", "average": null }, { "sample": "Plasma", "range": "94 - 104 %", "average": null }, { "sample": "Urine", "range": "94 - 102 %", "average": null } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab137976-1x96Tests", "assetComponentDetails": [ { "size":"1 x 150 µL", "name":"40X Biotinylated Human Factor V Antibody", "number":"AB137976-CMP05", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB137976-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Factor V Standard", "number":"AB137976-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Factor V Microplate (12 x 8 well strips)", "number":"AB137976-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB137976-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB137976-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB137976-CMP08", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB137976-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB137976-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor V also known as proaccelerin is a coagulation factor playing a main role in blood clotting. This protein with a molecular mass of approximately 330 kDa is synthesized in the liver and found in plasma. The Factor V protein is essential for the coagulation cascade functioning as a pivotal mediator in the conversion of prothrombin to thrombin. It exists in plasma in an inactive form until activated by thrombin or factor Xa to fulfill its mechanical function in hemostasis.
Biological function summary

The mechanism of Factor V involves its function as a cofactor to Factor Xa in the prothrombinase complex. This complex formation enhances the efficiency of thrombin generation considerably accelerating clot formation. Factor V once converted to its active form aids in forming a stable clot by bridging and immobilizing cellular elements and fibrin strands. Expression occurs mainly in the liver but it also manifests in platelets where it enhances thrombin production during platelet activation and aggregation.

Pathways

The contribution of Factor V to coagulation is central within the hemostasis pathway specifically the intrinsic and extrinsic pathways of the coagulation cascade. Factor V binds with Factor X to form the prothrombinase complex which is important for the conversion of prothrombin to thrombin. Other proteins such as Factor VIII interact within this pathway to ensure blood clots form properly. Any disturbances in Factor V activity can significantly alter the normal flow of these pathways affecting thrombin generation and subsequent clotting.

Factor V mutations or deficiencies associate with conditions like Factor V Leiden and Factor V deficiency. Factor V Leiden is a genetic mutation that elevates the risk of thrombophilia leading to increased clot formation in veins. On the other hand a deficiency in Factor V can lead to Parahemophilia characterized by a tendency for excessive bleeding. In both conditions the abnormal activity of Factor V affects the interaction with proteins in the coagulation cascade especially impacting the prothrombinase complex and Factor X.
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Product protocols

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Target data

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
See full target information F5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:103971 PubMed35224470

2022

Coagulation factor V is a T-cell inhibitor expressed by leukocytes in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Prasanti Kotagiri,Paul A Lyons,Rafia S Al-Lamki,Federica Mescia,Laura Bergamaschi,Lorinda Turner,Michael D Morgan,Fernando J Calero-Nieto,Karsten Bach,Nicole Mende,Nicola K Wilson,Emily R Watts,Patrick H Maxwell,Patrick F Chinnery,Nathalie Kingston,Sofia Papadia,Kathleen E Stirrups,Neil Walker,Ravindra K Gupta,David K Menon,Kieren Allinson,Sarah J Aitken,Mark Toshner,Michael P Weekes,James A Nathan,Sarah R Walmsley,Willem H Ouwehand,Mary Kasanicki,Berthold Göttgens,John C Marioni,Kenneth G C Smith,Jordan S Pober,John R Bradley

Thrombosis research 200:91-98 PubMed33571724

2021

Procoagulant activity in high grade serous ovarian cancer patients following neoadjuvant chemotherapy-The role of the activated protein C pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mark P Ward,Feras Abu Saadeh,Sharon A O'Toole,John J O'Leary,Noreen Gleeson,Lucy A Norris
View all publications
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Product promise

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