AB323697

Human Factor V ELISA Kit

Recombinant
SimpleStep
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Human Factor V ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor V with a sensitivity of 166 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Coagulation factor V, Activated protein C cofactor, F5

7 Images

Sandwich ELISA - Human Factor V ELISA Kit (AB323697)

sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB323697)

Example of human Factor V standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB323697)

Interpolated concentration of native Factor V was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 1,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB323697)

Interpolated concentration of native Factor V was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 500 human serum and rhesus monkey serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB323697)

Interpolated concentration of native Factor V was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Hep G2 supernatant 10% and human CSF 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB323697)

Example of reactivity of human Factor V kit standard and natively purified human Factor Va (ab62415) standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed. The kit recognizes the light chain present in both native human Factor V and Factor Va.

sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB323697)

Example of human Factor V standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Factor V ELISA Kit (AB323697)

Interpolated concentration of native Factor V was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 1 : 1,000, plasma (citrate) 1 : 500, plasma (EDTA) 1 : 500, and plasma (heparin) 1 : 500. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 166 pg/mL

Range

468.75 - 30000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Factor V SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor V protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine, Saliva, Cerebral Spinal Fluid. Quantitate Human Factor V with 166 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.2" } ]

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Recovery

[ { "sample": "Serum", "range": "103 - 108 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "81 - 92 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "103 - 117 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "91 - 102 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "94 - 108 %", "average": "= 99" }, { "sample": "Urine", "range": "80 - 89 %", "average": "= 84" }, { "sample": "Saliva", "range": "89 - 102 %", "average": "= 96" }, { "sample": "Cerebral Spinal Fluid", "range": "98 - 111 %", "average": "= 106" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

See full target information F5

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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