Human Factor V ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor V with a sensitivity of 166 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 468.75 - 30000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 166 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
Alternative names
Coagulation factor V, Activated protein C cofactor, F5
What's included?
Human Factor V ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor V with a sensitivity of 166 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 468.75 - 30000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 166 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 6.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 3.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 106 | Range 103 - 108 % |
Sample type Citrate plasma | Average % = 87 | Range 81 - 92 % |
Sample type EDTA Plasma | Average % = 110 | Range 103 - 117 % |
Sample type Heparin Plasma | Average % = 95 | Range 91 - 102 % |
Sample type Cell culture supernatant | Average % = 99 | Range 94 - 108 % |
Sample type Urine | Average % = 84 | Range 80 - 89 % |
Sample type Saliva | Average % = 96 | Range 89 - 102 % |
Sample type Cerebral Spinal Fluid | Average % = 106 | Range 98 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Factor V SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor V protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine, Saliva, Cerebral Spinal Fluid. Quantitate Human Factor V with 166 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Sandwich ELISA - Human Factor V ELISA Kit (ab323697)
Example of reactivity of human Factor V kit standard and natively purified human Factor Va (Native Human Factor Va protein ab62415) standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed. The kit recognizes the light chain present in both native human Factor V and Factor Va.
Sandwich ELISA - Human Factor V ELISA Kit (ab323697)
Interpolated concentration of native Factor V was measured in duplicate at different sample concentrations. Undiluted samples are as follows: 1:500 human serum and rhesus monkey serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Factor V ELISA Kit (ab323697)
Interpolated concentration of native Factor V was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:1,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Factor V ELISA Kit (ab323697)
Example of human Factor V standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Factor V ELISA Kit (ab323697)
Interpolated concentration of native Factor V was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Hep G2 supernatant 10% and human CSF 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Factor V ELISA Kit (ab323697)
Interpolated concentration of native Factor V was measured in duplicate at different sample concentrations. Undiluted samples are as follows: human serum 1:1,000, plasma (citrate) 1:500, plasma (EDTA) 1:500, and plasma (heparin) 1:500. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Factor V ELISA Kit (ab323697)
Example of human Factor V standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
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