Human Factor VII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor VII with a sensitivity of 0.16 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Journal of gastroenterology and hepatology 34:1851-18592019Establishment of gene-edited pigs expressing human blood-coagulation factor VII and albumin for bioartificial liver use.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Li,Hongyi Meng,Qingjian Zou,Jianmin Zhang,Lei Cai,Bin Yang,Jun Weng,Liangxue Lai,Huaqiang Yang,Yi GaoPubMed 30884543
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.39 - 25 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.16 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
Alternative names
Coagulation factor VII, Proconvertin, Serum prothrombin conversion accelerator, SPCA, F7
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Human Factor VII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor VII with a sensitivity of 0.16 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.39 - 25 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 0.16 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 6.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 4 | C.V. 6.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 91 | Range 80 - 93 % |
Sample type Serum | Average % = 89 | Range 82 - 96 % |
Sample type EDTA Plasma | Average % = 85 | Range 80 - 93 % |
Sample type Heparin Plasma | Average % = 91 | Range 83 - 97 % |
Sample type Citrate plasma | Average % = 101 | Range 92 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Factor VII ELISA Kit (ab190810) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor VII protein in cit plasma, hep plasma, serum, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Factor VII with 292 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Factor VII is a plasma serine protease involved in the coagulation cascade. Processed in the liver, it circulates in the bloodstream as a zymogen. During coagulation it is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin. Factor VIIa can then convert factor IX to IXa in the presence of tissue factor and calcium.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Factor VII also known as factor 7 or factor seven is a plasma glycoprotein with a molecular mass of approximately 50 kDa. It plays an important role in the blood coagulation process. Factor VII is mainly produced in the liver and requires vitamin K for its synthesis. In the inactive form it circulates in the plasma but upon activation to factor 7a it initiates the extrinsic pathway of blood coagulation. This activation is a critical step in hemostasis which helps to prevent excessive bleeding.
- Biological function summary
Factor 7a indirectly activates further components in the blood coagulation cascade. Upon activation factor 7a becomes a part of the tissue factor (TF) complex which includes tissue factor expressed on subendothelial cells. The complex triggers the activation of factor IX and factor X propagating the coagulation cascade downstream. This series of activations amplifies the signal for clot formation highlighting factor VII's integral role in initiating coagulation.
- Pathways
Factor VII is primarily involved in the extrinsic pathway of the coagulation cascade. This pathway is initiated when factor 7a forms a complex with tissue factor a protein that becomes exposed at injury sites. Factor VII is also indirectly linked to the common coagulation pathway through activation of factor X. The interaction between these pathways ensures efficient and rapid clot formation. Additionally factor IX also interacts with factor VII in the intricate network of coagulation processes that sustain hemostasis.
- Associated diseases and disorders
Deficiencies or dysfunctions in factor VII can lead to coagulopathies such as congenital factor VII deficiency which results in excessively prolonged bleeding. Conversely elevated factor VII activity can contribute to thromboembolic disorders like myocardial infarction. In these conditions factor VII often interacts with proteins like factor X and tissue factor highlighting their clinical significance in managing blood coagulation abnormalities.
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3 product images
Sandwich ELISA - Human Factor VII ELISA Kit (ab190810)
Example of human FVII standard curve in Sample Diluent NS + Enhancer.
The FVII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Factor VII ELISA Kit (ab190810)
Interpolated concentrations of native FVII in human serum and plasma samples.
The concentrations of FVII were measured in duplicates, interpolated from the FVII standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (EDTA) 2.5%, plasma (citrate) 5% and serum (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FVII concentration was determined to be 513 ng/mL in serum, 642 ng/mL in plasma (EDTA), 368 ng/mL in plasma (citrate), and 517 ng/mL in plasma (heparin).
Sandwich ELISA - Human Factor VII ELISA Kit (ab190810)
Serum from eight individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FVII concentration was determined to be 536 ng/mL with a range of 316 – 800 ng/mL.
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