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AB168545

Human Factor VII ELISA Kit (with plasma controls)

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(2 Publications)

Human Factor VII ELISA Kit (with plasma controls) is a Sandwich (quantitative) ELISA for the measurement of Human Factor VII (with plasma controls) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Coagulation factor VII, Proconvertin, Serum prothrombin conversion accelerator, SPCA, F7

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Sandwich ELISA - Human Factor VII ELISA Kit (with plasma controls) (AB168545)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor VII ELISA Kit (with plasma controls) (AB168545)

Representative Standard Curve using ab168545

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

> 0.23 ng/mL

Range

2 - 22.5 ng/mL

Assay time

4h

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Reactivity data

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Product details

Abcam's Factor VII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII levels in cell culture supernatants, serum and plasma.

A Factor VII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor VII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor VII captured in plate.

Get results in 90 minutes with Human Factor VII ELISA Kit (ab190810) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VII also known as factor 7 or factor seven is a plasma glycoprotein with a molecular mass of approximately 50 kDa. It plays an important role in the blood coagulation process. Factor VII is mainly produced in the liver and requires vitamin K for its synthesis. In the inactive form it circulates in the plasma but upon activation to factor 7a it initiates the extrinsic pathway of blood coagulation. This activation is a critical step in hemostasis which helps to prevent excessive bleeding.
Biological function summary

Factor 7a indirectly activates further components in the blood coagulation cascade. Upon activation factor 7a becomes a part of the tissue factor (TF) complex which includes tissue factor expressed on subendothelial cells. The complex triggers the activation of factor IX and factor X propagating the coagulation cascade downstream. This series of activations amplifies the signal for clot formation highlighting factor VII's integral role in initiating coagulation.

Pathways

Factor VII is primarily involved in the extrinsic pathway of the coagulation cascade. This pathway is initiated when factor 7a forms a complex with tissue factor a protein that becomes exposed at injury sites. Factor VII is also indirectly linked to the common coagulation pathway through activation of factor X. The interaction between these pathways ensures efficient and rapid clot formation. Additionally factor IX also interacts with factor VII in the intricate network of coagulation processes that sustain hemostasis.

Deficiencies or dysfunctions in factor VII can lead to coagulopathies such as congenital factor VII deficiency which results in excessively prolonged bleeding. Conversely elevated factor VII activity can contribute to thromboembolic disorders like myocardial infarction. In these conditions factor VII often interacts with proteins like factor X and tissue factor highlighting their clinical significance in managing blood coagulation abnormalities.
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Product protocols

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Target data

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
See full target information F7
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 10:2005 PubMed32029851

2020

Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Clay T Cohen,Nancy A Turner,Joel L Moake

The international journal of biochemistry & cell b 113:67-74 PubMed31185295

2019

N-Glycan-calnexin interactions in human factor VII secretion and deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Lina Wang,Shuo Li,Ningzheng Dong,Qingyu Wu
View all publications
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websiteProtocolBooklet
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