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AB289650

Human Factor X ELISA Kit

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Human Factor X ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor X with a sensitivity of 14.63 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Coagulation factor X, Stuart factor, Stuart-Prower factor, F10

4 Images
Sandwich ELISA - Human Factor X ELISA Kit (AB289650)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor X ELISA Kit (AB289650)

Interpolated concentrations of human Factor X in HepG2 supernatant, saliva, and cerebrospinal fluid.

Interpolated concentration of native Factor X was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 supernatant 1 : 80, male saliva 50%, pooled sex cerebrospinal fluid 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor X ELISA Kit (AB289650)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor X ELISA Kit (AB289650)

Example of human Factor X standard curve in Sample Diluent NS.

Example of human Factor X standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor X ELISA Kit (AB289650)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor X ELISA Kit (AB289650)

Interpolated concentrations of human Factor X in serum, plasma (citrate), plasma (EDTA), and cell culture supernatant.

Interpolated concentration of native Factor X was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 4,000, and plasma (EDTA) 1 : 4,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor X ELISA Kit (AB289650)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor X ELISA Kit (AB289650)

Interpolated concentrations of Factor X in normal human serum donors.

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor X concentration was determined to be 11.73 μg/mL with a range of 7.21 – 15.93 μg/mL

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 14.63 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Factor X ELISA Kit (ab289650) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Factor X in serum, plasma (citrate), plasma (EDTA), cell culture supernatant, urine, milk, saliva, and cerebrospinal fluid samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Factor X with 14.63 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 122 %", "average": "= 107" }, { "sample": "Milk", "range": "89 - 95 %", "average": "= 92" }, { "sample": "Serum", "range": "97 - 101 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "87 - 93 %", "average": "= 90" }, { "sample": "Cerebral Spinal Fluid", "range": "95 - 97 %", "average": "= 96" }, { "sample": "Saliva", "range": "97 - 102 %", "average": "= 98" }, { "sample": "Urine", "range": "96 - 107 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "99 - 112 %", "average": "= 104" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor X also known as Stuart-Prower Factor is a critical component in the coagulation cascade. It has a molecular weight of approximately 59 kDa and is expressed mainly in the liver. This serine protease plays an important role in the conversion of prothrombin to thrombin an essential step in blood clot formation. Factor X circulates in the plasma as an inactive zymogen and becomes activated to its enzyme form factor Xa in response to specific physiological signals.
Biological function summary

Factor X contributes significantly to the coagulation process by activating prothrombin into thrombin. It is not part of a larger complex but functions closely with other components of the coagulation cascade to maintain hemostasis. Factor X interacts with calcium ions and phospholipids on the surface of platelets enhancing its enzymatic activity. This activation ensures proper regulation of blood clotting preventing excessive bleeding or uncontrolled clot formation.

Pathways

Factor X is central to both the intrinsic and extrinsic coagulation pathways. It interacts with proteins such as factor VIIa in the extrinsic pathway and factor IXa in the intrinsic pathway both pathways leading to the activation of factor Xa. In turn factor Xa is key in the common pathway which combines inputs from the intrinsic and extrinsic pathways to produce thrombin essential for fibrin clot formation.

Factor X is associated with bleeding disorders such as hemophilia and liver disease. Deficiencies or dysfunctions in factor X result in inadequate clot formation leading to prolonged bleeding episodes. Furthermore increased factor X activity as in cases of thrombophilia can raise the risk of abnormal blood clots. Factor X works alongside proteins like factor V with both deficiencies playing roles in hemophilia. Monitoring factor X activity is important in diagnosing and managing these coagulation-related conditions.
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Product protocols

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Target data

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).
See full target information F10
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