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AB168547

Human Factor XI ELISA Kit (with plasma controls)

4

(1 Review)

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(1 Publication)

Human Factor XI ELISA Kit (with plasma controls) is a Sandwich (quantitative) ELISA for the measurement of Human Factor XI (with plasma controls) in Human in Biofluids samples.

View Alternative Names

Coagulation factor XI, FXI, Plasma thromboplastin antecedent, PTA, F11

1 Images
Sandwich ELISA - Human Factor XI ELISA Kit (with plasma controls) (AB168547)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (with plasma controls) (AB168547)

Representative Standard Curve using ab168547

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

> 0.75 ng/mL

Range

1.563 - 50 ng/mL

Assay time

4h

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Factor XI Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XI concentrations in human plasma and serum.

A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 106 %", "average": null }, { "sample": "Plasma", "range": "94 - 105 %", "average": null } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XI (FXI) also known as Factor 11 or Factor Eleven is an important protein in the human body involved in blood coagulation. It has a molecular mass of approximately 160 kDa and is primarily expressed in the liver. Factor XI circulates in the blood as a zymogen an inactive enzyme precursor until it is activated during the coagulation process. Its activation is a critical step in the chain of events leading to blood clot formation. FXI can be measured using assays such as ELISA and it is often used in laboratory settings to study coagulation mechanics.
Biological function summary

FXI plays a significant role in the intrinsic pathway of the coagulation cascade. It forms a complex with high-molecular-weight kininogen and prekallikrein on the surface of activated platelets ensuring that the coagulation process proceeds efficiently. Upon activation by Factor XIIa FXI itself becomes an active serine protease known as FXIa which then converts Factor IX into its active form Factor IXa. This step highlights the importance of FXI in amplifying the coagulation process ensuring rapid and effective hemostasis.

Pathways

Factor XI is deeply integrated into the intrinsic coagulation cascade. It works alongside other proteins such as Factor XII and Factor IX to facilitate the formation of a blood clot. The intrinsic pathway is essential for maintaining hemostasis particularly in response to internal injuries within the blood vessels. Its interaction with these proteins allows for a powerful amplification of the clotting signal ensuring a robust clot formation and effectively sealing vascular injuries.

Dysfunction or deficiency in FXI can lead to bleeding disorders such as Hemophilia C although this is less common than Hemophilia A or B. Interestingly elevated levels of FXI are sometimes associated with an increased risk of thrombotic conditions such as deep vein thrombosis or stroke. In these conditions FXI interacts with other coagulation factors like Factor IX and Factor XII highlighting its dual role in both bleeding and clotting disorders. Understanding FXI's role provides insight into therapeutic targets for managing these conditions.
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Product protocols

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Target data

Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
See full target information F11
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of thrombosis and haemostasis : JTH 19:920-930 PubMed33421272

2021

Essential role of a carboxyl-terminal α-helix motif in the secretion of coagulation factor XI.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri Hayakawa,Shogo Tamura,Nobuaki Suzuki,Koya Odaira,Mahiru Tokoro,Fumika Kawashima,Fumihiko Hayakawa,Akira Takagi,Akira Katsumi,Atsuo Suzuki,Shuichi Okamoto,Takeshi Kanematsu,Tadashi Matsushita,Tetsuhito Kojima
View all publications
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websiteProtocolBooklet
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