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AB108835

Human Factor XII ELISA Kit

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(2 Publications)

Human Factor XII ELISA Kit is a sandwich ELISA designed to quantify Human Factor XII with a sensitivity of 49 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader

View Alternative Names

Coagulation factor XII, Hageman factor, HAF, F12

4 Images
ELISA - Human Factor XII ELISA Kit (AB108835)
  • ELISA

Lab

ELISA - Human Factor XII ELISA Kit (AB108835)

Standard curve with background signal subtracted (duplicates; +/- SD).

ELISA - Human Factor XII ELISA Kit (AB108835)
  • ELISA

Lab

ELISA - Human Factor XII ELISA Kit (AB108835)

Factor XII measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested in the dilution range of 1/100-1/4000; milk, saliva, CSF and urine were diluted 1/1-1/300.

ELISA - Human Factor XII ELISA Kit (AB108835)
  • ELISA

Lab

ELISA - Human Factor XII ELISA Kit (AB108835)

Factor XII measured in undiluted culture supernatants, MCF-7 levels below level of detection (duplicates +/- SD).

Sandwich ELISA - Human Factor XII ELISA Kit (AB108835)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB108835)

Representative Standard Curve using ab108835

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Milk, Plasma, Serum, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 49 pg/mL

Range

0.098 - 100 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Factor XII ELISA Kit ab108835 is a sandwich ELISA to measure Human Factor XII in serum, plasma, CSF, urine, milk with a sensitivity of 49 pg/ml.

How the assay works

A Factor XII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XII captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Factor XII ELISA Kit ab108835 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit

Human Factor XII ELISA Kit (ab192144
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.7" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XII also known as FXII or Hageman factor is a serine protease and a coagulation factor that plays an important role in initiating the intrinsic pathway of blood coagulation. It has a molecular mass of approximately 80 kDa. The liver primarily synthesizes this protein and it circulates in the blood plasma as an inactive precursor. When activated to Factor XIIa it cleaves and activates other coagulation factors such as Factor XI to propagate the coagulation cascade that helps in blood clot formation.
Biological function summary

This protein helps control blood coagulation processes as part of a larger network of interacting proteins that includes contact activation factors. Factor XII activates not only the coagulation cascade but also pathways involved in fibrinolysis inflammation and bradykinin formation. It triggers the kallikrein-kinin system and leads to the generation of bradykinin a peptide known to induce vascular permeability and inflammation.

Pathways

The involvement of Factor XII spans the intrinsic coagulation pathway and the kallikrein-kinin system. It interacts with several other coagulation factors such as Factor XI and prekallikrein to advance the clotting process. The protein initiates the intrinsic blood coagulation pathway by converting Factor XI to Factor XIa in the presence of negatively charged surfaces. It links to the fibrinolytic pathway and can indirectly activate plasminogen through interactions that generate plasmin.

Factor XII deficiency leads to increased risk of thrombosis even though such deficiency generally shows no major bleeding symptoms. The target links to hereditary angioedema where dysregulation in the kallikrein-kinin system involves premature bradykinin activity. Factor XII associates with C1-inhibitor a protein playing a regulatory role in preventing excessive bradykinin production which can manifest in clinical symptoms seen in hereditary angioedema patients.
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Product protocols

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Target data

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (PubMed : 2019570, PubMed : 21304106, PubMed : 8427954).
See full target information F12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 30:105565 PubMed33412399

2021

Factor XII Concentrations and Risk of Intracerebral Haemorrhage. A Prospective Case-Referent Study.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Johansson,Lars Johansson,Torbjörn K Nilsson,Marcus M Lind

Oncotarget 9:14862-14881 PubMed29599912

2018

Coagulation cascade and complement system in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Shang-Bo Xie,Chang-Hao Wu,Yuan Hu,Qin Zhang,Si Li,Yin-Guang Fan,Rui-Xue Leng,Hai-Feng Pan,Hua-Bao Xiong,Dong-Qing Ye
View all publications
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Product promise

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