JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB192144

Human Factor XII ELISA Kit

Be the first to review this product! Submit a review

|

(2 Publications)

Human Factor XII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor XII with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Coagulation factor XII, Hageman factor, HAF, F12

7 Images
ELISA - Human Factor XII ELISA Kit (AB192144)
  • ELISA

Supplier Data

ELISA - Human Factor XII ELISA Kit (AB192144)
Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)
  • sELISA

Lab

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 6,600 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)
  • sELISA

Lab

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Example of human Factor XII standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 30.4 μg/mL with a range of 21.8 – 40.3 μg/mL.

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Interpolated concentrations of native Factor XII in human urine samples.

The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 60.7 ng/mL in urine 12.5%.

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Interpolated concentrations of native Factor XII in human serum, plasma and cell culture supernatant samples.

The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 6,600, plasma (EDTA) 1 : 1,600, plasma (citrate) 1 : 1,600 and plasma (heparin) 1 : 8,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 25.0 μg/mL in serum, 21.7 μg/mL in plasma (EDTA), 21.0 μg/mL in plasma (citrate), and 32.1 μg/mL in plasma (heparin).

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Example of human Factor XII standard curve in Sample Diluent NS.

The Factor XII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.1 ng/mL

Range

0.23 - 15 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Factor XII ELISA Kit ab192144 is a rapid single-wash 90-min Sandwich ELISA to measure Human Factor XII in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 0.1 ng/mL.

How the assay works

Human Factor XII SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Factor XII ELISA Kit ab192144 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human Factor XII ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Factor XII antibody [EPR22292-151] - BSA and Azide free (Capture) ab259448
- Anti-Factor XII antibody [EPR22292-25] - BSA and Azide free (Detector) ab259448

Factor XII is a 80 kDa plasma serine protease involved in the coagulation cascade. Factor XII is activated by proteolysis and the product, Factor XIIa, goes on to activate Factor XI and prekallikrein in in vitro models of blood cogulation. Its involvement in the beginning of the coagulation cascade is only seen in in vitro studies, and is thought to have no effect on coagulation initiation in vivo. While Factor XII deficiency in humans is asymptomatic, Factor XII plays a role in later stages of clot formation. Deficiency of Factor XII affects the in vitro measurements of partial thromboplastin time in human blood, and its absence decreases the susceptibility of mice to thrombosis.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 98 %", "average": "= 93" }, { "sample": "Serum", "range": "98 - 118 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "117 - 123 %", "average": "= 120" }, { "sample": "Urine", "range": "104 - 110 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "100 - 109 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "108 - 116 %", "average": "= 110" } ]
style
left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab192144-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB192144-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB192144-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB192144-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB192144-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human Factor XII Capture Antibody", "number":"AB192144-CMP01" }, { "size":"2 x 1 Vial", "name":"Human Factor XII Lyophilized Purified Protein (ab62423)", "number":"AB192144-CMP04" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB192144-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human Factor XII Detector Antibody", "number":"AB192144-CMP02" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB192144-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB192144-CMP11" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab192144-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB192144-CMP07" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB192144-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB192144-CMP08" }, { "size":"2 x 10 Vial", "name":"Human Factor XII Lyophilized Purified Protein (ab62423)", "number":"AB192144-CMP04" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB192144-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB192144-CMP03" }, { "size":"1 x 6000 µL", "name":"10X Human Factor XII Capture Antibody", "number":"AB192144-CMP01" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB192144-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB192144-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Human Factor XII Detector Antibody", "number":"AB192144-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab192144-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB192144-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB192144-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB192144-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB192144-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB192144-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB192144-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Factor XII Lyophilized Purified Protein (ab62423)", "number":"AB192144-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Factor XII Capture Antibody", "number":"AB192144-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Factor XII Detector Antibody", "number":"AB192144-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB192144-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XII also known as FXII or Hageman factor is a serine protease and a coagulation factor that plays an important role in initiating the intrinsic pathway of blood coagulation. It has a molecular mass of approximately 80 kDa. The liver primarily synthesizes this protein and it circulates in the blood plasma as an inactive precursor. When activated to Factor XIIa it cleaves and activates other coagulation factors such as Factor XI to propagate the coagulation cascade that helps in blood clot formation.
Biological function summary

This protein helps control blood coagulation processes as part of a larger network of interacting proteins that includes contact activation factors. Factor XII activates not only the coagulation cascade but also pathways involved in fibrinolysis inflammation and bradykinin formation. It triggers the kallikrein-kinin system and leads to the generation of bradykinin a peptide known to induce vascular permeability and inflammation.

Pathways

The involvement of Factor XII spans the intrinsic coagulation pathway and the kallikrein-kinin system. It interacts with several other coagulation factors such as Factor XI and prekallikrein to advance the clotting process. The protein initiates the intrinsic blood coagulation pathway by converting Factor XI to Factor XIa in the presence of negatively charged surfaces. It links to the fibrinolytic pathway and can indirectly activate plasminogen through interactions that generate plasmin.

Factor XII deficiency leads to increased risk of thrombosis even though such deficiency generally shows no major bleeding symptoms. The target links to hereditary angioedema where dysregulation in the kallikrein-kinin system involves premature bradykinin activity. Factor XII associates with C1-inhibitor a protein playing a regulatory role in preventing excessive bradykinin production which can manifest in clinical symptoms seen in hereditary angioedema patients.
style
left-column

Product protocols

style
left-column

Target data

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (PubMed : 2019570, PubMed : 21304106, PubMed : 8427954).
See full target information F12
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of thrombosis and haemostasis : JTH 19:2835-2840 PubMed34363738

2021

Generation of a humanized FXII knock-in mouse-A powerful model system to test novel anti-thrombotic agents.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Beck,David Stegner,Stefan Loroch,Ayesha A Baig,Vanessa Göb,Cornelia Schumbrutzki,Eva Eilers,Albert Sickmann,Frauke May,Marc W Nolte,Con Panousis,Bernhard Nieswandt

Transplantation 103:1821-1833 PubMed30964836

2019

Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A Fernandez
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB108834

Human Factor XI ELISA Kit

Sandwich ELISA - Human Factor XI ELISA Kit (AB108834)

  • 0
  • 0
Assay Kits

AB287172

Human Factor XIIIa ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Factor XIIIa ELISA Kit (AB287172)

  • 0
  • 0
Assay Kits

AB223864

Human Von Willebrand Factor ELISA Kit

Recombinant|SimpleStep

ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)

  • 0
  • 0
Assay Kits

AB190810

Human Factor VII ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Factor VII ELISA Kit (AB190810)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com