Human Factor XII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor XII with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Journal of thrombosis and haemostasis : JTH 19:2835-28402021Generation of a humanized FXII knock-in mouse-A powerful model system to test novel anti-thrombotic agents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Beck,David Stegner,Stefan Loroch,Ayesha A Baig,Vanessa Göb,Cornelia Schumbrutzki,Eva Eilers,Albert Sickmann,Frauke May,Marc W Nolte,Con Panousis,Bernhard NieswandtPubMed 34363738
- Transplantation 103:1821-18332019Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A FernandezPubMed 30964836
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.23 - 15 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (PubMed:2019570, PubMed:21304106, PubMed:8427954).
Alternative names
Coagulation factor XII, Hageman factor, HAF, F12
What's included?
Recommended products
Human Factor XII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor XII with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.23 - 15 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 0.1 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 5.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 93 | Range 88 - 98 % |
Sample type Serum | Average % = 109 | Range 98 - 118 % |
Sample type EDTA Plasma | Average % = 120 | Range 117 - 123 % |
Sample type Urine | Average % = 107 | Range 104 - 110 % |
Sample type Heparin Plasma | Average % = 105 | Range 100 - 109 % |
Sample type Citrate plasma | Average % = 110 | Range 108 - 116 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Factor XII ELISA Kit (ab192144) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor XII protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Factor XII with 0.1 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Factor XII is a 80 kDa plasma serine protease involved in the coagulation cascade. Factor XII is activated by proteolysis and the product, Factor XIIa, goes on to activate Factor XI and prekallikrein in in vitro models of blood cogulation. Its involvement in the beginning of the coagulation cascade is only seen in in vitro studies, and is thought to have no effect on coagulation initiation in vivo. While Factor XII deficiency in humans is asymptomatic, Factor XII plays a role in later stages of clot formation. Deficiency of Factor XII affects the in vitro measurements of partial thromboplastin time in human blood, and its absence decreases the susceptibility of mice to thrombosis.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Factor XII also known as FXII or Hageman factor is a serine protease and a coagulation factor that plays an important role in initiating the intrinsic pathway of blood coagulation. It has a molecular mass of approximately 80 kDa. The liver primarily synthesizes this protein and it circulates in the blood plasma as an inactive precursor. When activated to Factor XIIa it cleaves and activates other coagulation factors such as Factor XI to propagate the coagulation cascade that helps in blood clot formation.
- Biological function summary
This protein helps control blood coagulation processes as part of a larger network of interacting proteins that includes contact activation factors. Factor XII activates not only the coagulation cascade but also pathways involved in fibrinolysis inflammation and bradykinin formation. It triggers the kallikrein-kinin system and leads to the generation of bradykinin a peptide known to induce vascular permeability and inflammation.
- Pathways
The involvement of Factor XII spans the intrinsic coagulation pathway and the kallikrein-kinin system. It interacts with several other coagulation factors such as Factor XI and prekallikrein to advance the clotting process. The protein initiates the intrinsic blood coagulation pathway by converting Factor XI to Factor XIa in the presence of negatively charged surfaces. It links to the fibrinolytic pathway and can indirectly activate plasminogen through interactions that generate plasmin.
- Associated diseases and disorders
Factor XII deficiency leads to increased risk of thrombosis even though such deficiency generally shows no major bleeding symptoms. The target links to hereditary angioedema where dysregulation in the kallikrein-kinin system involves premature bradykinin activity. Factor XII associates with C1-inhibitor a protein playing a regulatory role in preventing excessive bradykinin production which can manifest in clinical symptoms seen in hereditary angioedema patients.
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6 product images
Sandwich ELISA - Human Factor XII ELISA Kit (ab192144)
Example of human Factor XII standard curve in Sample Diluent NS.
The Factor XII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Factor XII ELISA Kit (ab192144)
Interpolated concentrations of native Factor XII in human serum, plasma and cell culture supernatant samples.
The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:6,600, plasma (EDTA) 1:1,600, plasma (citrate) 1:1,600 and plasma (heparin) 1:8,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 25.0 μg/mL in serum, 21.7 μg/mL in plasma (EDTA), 21.0 μg/mL in plasma (citrate), and 32.1 μg/mL in plasma (heparin).
Sandwich ELISA - Human Factor XII ELISA Kit (ab192144)
Interpolated concentrations of native Factor XII in human urine samples.
The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 60.7 ng/mL in urine 12.5%.
Sandwich ELISA - Human Factor XII ELISA Kit (ab192144)
Serum from eight individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 30.4 μg/mL with a range of 21.8 – 40.3 μg/mL.
Sandwich ELISA - Human Factor XII ELISA Kit (ab192144)
Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:6,600 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Factor XII ELISA Kit (ab192144)
Example of human Factor XII standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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