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AB287172

Human Factor XIIIa ELISA Kit

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Human Factor XIIIa ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor XIIIa with a sensitivity of 0.092 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

F13A, F13A1, Coagulation factor XIII A chain, Coagulation factor XIIIa, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain

3 Images
Sandwich ELISA - Human Factor XIIIa ELISA Kit (AB287172)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XIIIa ELISA Kit (AB287172)

Example of human Factor XIIIa standard curve in Sample Diluent NS + 1X Enhancer.

Example of human Factor XIIIa standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor XIIIa ELISA Kit (AB287172)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XIIIa ELISA Kit (AB287172)

Representative image of Human Factor XIIIa ELISA Kit (ab287172)

Components shown from left to right :

- Sample diluent NS (2 bottles)

- Denaturant

- 10X Human Factor XIIIa Capture Antibody

- 10X Human Factor XIIIa Detector Antibody

- Human Factor XIIIa Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Factor XIIIa ELISA Kit (AB287172)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XIIIa ELISA Kit (AB287172)

Interpolated concentrations of human Factor XIIIa in serum, plasma (citrate), plasma (EDTA), and plasma(heparin).

Interpolated concentration of native Factor XIIIa was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 400, plasma (EDTA) 1 : 400, and plasma (heparin) 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.092 ng/mL

Range

0.469 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Factor XIIIa ELISA kit (ab287172) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Factor XIIIa protein in human serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Factor XIIIa with 0.092 ng/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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Recovery

[ { "sample": "Serum", "range": "81 - 88 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "87 - 95 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "87 - 93 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "88 - 89 %", "average": "= 89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab287172-1x96Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB287172-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB287172-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB287172-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB287172-CMP13", "productcode":"" }, { "size":"1 x 100 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB287172-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB287172-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB287172-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB287172-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Factor XIIIa Lyophilized Recombinant Protein", "number":"AB287172-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Factor XIIIa Capture Antibody", "number":"AB287172-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Factor XIIIa Detector Antibody", "number":"AB287172-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB287172-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XIIIa also known as Factor 13a Factor XIII or Factor 13 is an enzyme that plays an important role in blood coagulation. Its mass is approximately 83 kDa. This enzyme is part of the transglutaminase family and is predominantly found in plasma and platelets. Factor XIIIa becomes active when thrombin and calcium ions cleave its A subunits converting it from an inactive zymogen known simply as Factor XIII or Factor 13 to its active form. The best assays for Factor XIIIa often measure its transglutaminase activity which facilitates the cross-linking of fibrin stabilizing blood clots.
Biological function summary

Factor XIIIa is important for hemostasis facilitating the final stages of blood clot formation by cross-linking fibrin polymers. This enzyme does not function in isolation; it forms a tetrameric complex with two A subunits and two B subunits. The active form Factor XIIIa catalyzes the formation of covalent bonds between glutamine and lysine residues in fibrin chains. This enhances the mechanical strength and resistance of the clot preventing premature degradation.

Pathways

Factor XIIIa is part of the coagulation pathway specifically involved in the stabilization of fibrin in the final steps of the cascade. It interacts closely with proteins like fibrinogen and thrombin as these proteins are essential in the conversion of Factor XIII to Factor XIIIa and in clot formation. Factor XIIIa also plays a role in the wound healing process by stabilizing the ECM (extracellular matrix) through cross-linking fibrin fibronectin and collagen.

Factor XIIIa deficiency can lead to bleeding diathesis a condition characterized by poor clot stability and prolonged bleeding. This deficiency is often congenital and involves reduced levels or activity of Factor XIII. Conversely overactivity or dysregulation of Factor XIIIa may contribute to thrombotic disorders where abnormal clot formation can lead to conditions like deep vein thrombosis. Clinically understanding Factor XIIIa interactions especially its functional relationships with fibrinogen and thrombin is critical in managing these bleeding and thrombotic disorders.
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Product protocols

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Target data

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
See full target information F13A1
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websiteProtocolBooklet
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