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AB314700

Human FAM19A5 ELISA Kit

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Human FAM19A5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human FAM19A5 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

FAM19A5, UNQ5208/PRO34524, TAFA5, Chemokine-like protein TAFA-5

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Sandwich ELISA - Human FAM19A5 ELISA Kit (AB314700)
  • sELISA

Supplier Data

Sandwich ELISA - Human FAM19A5 ELISA Kit (AB314700)

Example of standard curve using ab314700. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 15 pg/mL

Range

15 - 4000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FAM19A5 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FAM19A5 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human FAM19A5 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "125 - 147 %", "average": "= 136.5" }, { "sample": "Plasma", "range": "97 - 136 %", "average": "= 118.9" }, { "sample": "Cell culture media", "range": "116 - 139 %", "average": "= 125.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314700-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314700-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314700-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human FAM19A5 Detection Antibody", "number":"AB314700-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB314700-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314700-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314700-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314700-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human FAM19A5 Antibody-coated ELISA Plate", "number":"AB314700-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human FAM19A5 Protein Standard", "number":"AB314700-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FAM19A5 also known as TAFA5 is a member of the TAFA family and plays a role in the regulation of nervous system function. It has an approximate mass of 13 kDa and exhibits expression primarily in the central nervous system including regions such as the brain. This protein is predominantly secreted as a small cytokine-like protein where it is involved in intercellular communication within the nervous tissue.
Biological function summary

The FAM19A5 protein influences neuroinflammatory processes and modulates the activity of neurons. While it does not form part of a large protein complex it may interact with extracellular matrix components to exert its effects. Its function is linked to the modulation of neural signaling indicating its role in maintaining homeostasis within the neural networks.

Pathways

This protein is known to engage with critical signaling cascades implicated in the central nervous system. In particular FAM19A5 is associated with the regulation of the chemokine signaling pathway. It is linked to pathways also involving proteins such as CCR5 and CXCR3 which interact in chemotaxis and influence neural cell responses. These pathways help in communicating signals that guide neural cell behavior affecting motor coordination and cognitive functions.

Current research suggests the involvement of FAM19A5 in conditions like neurodegenerative diseases and multiple sclerosis (MS). Its function in modulating neuroinflammation connects it to these disorders which involve immune responses that damage neural tissues. FAM19A5 may interact with other immune-related proteins like cytokines contributing to the pathophysiological processes observed in these diseases. The study of FAM19A5 provides insights into therapeutic targets for the management of such neurological disorders.
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Product protocols

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Target data

Acts as a chemokine-like protein by regulating cell proliferation and migration through activation of G protein-coupled receptors (GPCRs), such as S1PR2 and FPR2 (By similarity). Stimulates chemotactic migration of macrophages mediated by the MAPK3/ERK1 and AKT1 pathway (By similarity). Blocks TNFSF11/RANKL-induced osteoclast formation from macrophages by inhibiting up-regulation of osteoclast fusogenic and differentiation genes (By similarity). Stimulation of macrophage migration and inhibition of osteoclast formation is mediated via GPCR FPR2 (By similarity). Acts as an adipokine by negatively regulating vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor stimulation via GPCR S1PR2 and G protein GNA12/GNA13-transmitted RHOA signaling (By similarity). Inhibits injury-induced cell proliferation and neointima formation in the femoral arteries (By similarity).
See full target information TAFA5
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websiteProtocolBooklet
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