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AB256404

Human FAP ELISA Kit

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Human FAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FAP with a sensitivity of 14.96 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, FAP

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ELISA - Human FAP ELISA Kit (AB256404)
  • ELISA

Supplier Data

ELISA - Human FAP ELISA Kit (AB256404)
Sandwich ELISA - Human FAP ELISA Kit (AB256404)
  • sELISA

Supplier Data

Sandwich ELISA - Human FAP ELISA Kit (AB256404)

2.5% Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAP concentration was determined to be 53.12 ng/mL with a range of 20.47 – 101.21 ng/mL.

Sandwich ELISA - Human FAP ELISA Kit (AB256404)
  • sELISA

Supplier Data

Sandwich ELISA - Human FAP ELISA Kit (AB256404)

Interpolated concentrations of native FAP in human serum and plasma samples.

The concentrations of FAP were measured in duplicates, interpolated from the FAP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAP concentration was determined to be 29.25 ng/mL in serum, 24.36 ng/mL in plasma (citrate), 26.29 ng/mL in plasma (EDTA), and 24.01 ng/mL in plasma (heparin).

Sandwich ELISA - Human FAP ELISA Kit (AB256404)
  • sELISA

Supplier Data

Sandwich ELISA - Human FAP ELISA Kit (AB256404)

Example of human FAP standard curve in Sample Diluent NS.

The FAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 14.96 pg/mL

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FAP ELISA Kit ab256404 is a rapid single-wash 90-min Sandwich ELISA to measure Human FAP in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 14.96 pg/mL.

How the assay works

Human FAP SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human FAP ELISA Kit ab256404 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Fibroblast activation protein, alpha antibody [EPR22284-138] - BSA and Azide free (Capture) ab259462
- Anti-Fibroblast activation protein, alpha antibody [EPR22284-169] - BSA and Azide free (Detector) ab259462

FAP (fibroblast activation protein-alpha; also known as seprase) is a 86.3 kD type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue. FAP may play roles in tumor invasion, tissue remodeling, and wound repair. Human FAP shares 90.1%, 89.5%, 93.9%, and 97.3% sequence homology with mouse, rat, bovine, and monkey, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 102 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "96 - 108 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "91 - 96 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "91 - 101 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "82 - 102 %", "average": "= 90" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed : 14751930, PubMed : 16223769, PubMed : 16410248, PubMed : 16480718, PubMed : 17381073, PubMed : 18095711, PubMed : 21288888, PubMed : 24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed : 10347120, PubMed : 10455171, PubMed : 12376466, PubMed : 16223769, PubMed : 16651416, PubMed : 18095711, PubMed : 2172980, PubMed : 7923219, PubMed : 9065413). Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed : 10347120, PubMed : 10593948, PubMed : 16175601, PubMed : 16223769, PubMed : 16410248, PubMed : 16651416, PubMed : 17381073, PubMed : 21314817, PubMed : 24371721, PubMed : 24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed : 21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
See full target information FAP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:2004168 PubMed34141520

2021

Mistranslation Drives Alterations in Protein Levels and the Effects of a Synonymous Variant at the Fibroblast Growth Factor 21 Locus.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Bayoumi,Asmaa Elsayed,Shuanglin Han,Salvatore Petta,Leon A Adams,Rocio Aller,Anis Khan,Carmelo García-Monzón,María Teresa Arias-Loste,Luca Miele,Olivier Latchoumanin,Shafi Alenizi,Rocio Gallego-Durán,Janett Fischer,Thomas Berg,Antonio Craxì,Mayada Metwally,Liang Qiao,Christopher Liddle,Hannele Yki-Järvinen,Elisabetta Bugianesi,Manuel Romero-Gomez,Jacob George,Mohammed Eslam
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Product promise

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