Human Fas Ligand ELISA Kit (APTL) is a Sandwich (quantitative) ELISA kit for the measurement of Human Fas Ligand (APTL) in Human in Plasma, Cell culture supernatant, Serum samples.
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- Microbiology spectrum 10:e01357222022Mixed T Helper1/T Helper2/T Cytotoxic Profile in Subjects with Chronic Chagas Disease with Hypersensitivity Reactions to Benznidazole.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelisa D Castro Eiro,María A Natale,María G Alvarez,Araceli Castro,Débora Seigelshifer,Rodolfo Viotti,Marisa Fernández,Luis Mazzuoccolo,Bruno Lococo,Graciela L Bertocchi,Gonzalo Cesar,María C Albareda,María J Elias,María B Caputo,Eduardo Gaddi,Jeanette Balbaryski,Carlos A Vigliano,Susana A LaucellaPubMed 35938810
- Frontiers in physiology 12:7088242021Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaola Ayala-Ramírez,Catalina Machuca-Acevedo,Tatiana Gámez,Sandra Quijano,Alfonso Barreto,Jaime L Silva,Mercedes Olaya-C,Reggie García-RoblesPubMed 34366896
- Journal of extracellular vesicles 10:e120222020Microglial derived extracellular vesicles activate autophagy and mediate multi-target signaling to maintain cellular homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBram Van den Broek,Isabel Pintelon,Ibrahim Hamad,Sofie Kessels,Mansour Haidar,Niels Hellings,Jerome J A Hendriks,Markus Kleinewietfeld,Bert Brône,Vincent Timmerman,Jean-Pierre Timmermans,Veerle Somers,Luc Michiels,Joy IrobiPubMed 33708355
- Frontiers in pediatrics 6:1392018Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Zhang et. al.PubMed 29977885
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.37 - 1000 pg/mL
- Sensitivity
- < 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:7528780, PubMed:9228058, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260). Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed:27806260). FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
Alternative names
CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL
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Human Fas Ligand ELISA Kit (APTL) is a Sandwich (quantitative) ELISA kit for the measurement of Human Fas Ligand (APTL) in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.37 - 1000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 2 pg/mL
- General recovery
- 75%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 76.99 | Range 70 - 90 % |
Sample type Serum | Average % = 76.36 | Range 70 - 87 % |
Sample type Plasma | Average % = 73.42 | Range 67 - 86 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Fas Ligand (APTL) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas Ligand in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
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2 product images
Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (ab100515)
Representative Standard Curve using ab100515.
Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (ab100515)
Representative Standard Curve using ab100515.
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