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AB100515

Human Fas Ligand ELISA Kit (APTL)

4

(2 Reviews)

|

(4 Publications)

Human Fas Ligand ELISA Kit (APTL) is a Sandwich (quantitative) ELISA for the measurement of Human Fas Ligand (APTL) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL

2 Images
Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)

Representative Standard Curve using ab100515.

Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)

Representative Standard Curve using ab100515.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

1.37 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fas Ligand (APTL) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas Ligand in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "70 - 90 %", "average": "= 76.99" }, { "sample": "Serum", "range": "70 - 87 %", "average": "= 76.36" }, { "sample": "Plasma", "range": "67 - 86 %", "average": "= 73.42" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100515-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100515-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100515-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100515-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100515-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100515-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fas Ligand Microplate (12 x 8 wells)", "number":"AB100515-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Fas Ligand Standard (lyophilized)", "number":"AB100515-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Fas Ligand", "number":"AB100515-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100515-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed : 26334989, PubMed : 9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed : 7528780, PubMed : 9228058, PubMed : 9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed : 27806260).. Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed : 27806260).. FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
See full target information FASLG
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum 10:e0135722 PubMed35938810

2022

Mixed T Helper1/T Helper2/T Cytotoxic Profile in Subjects with Chronic Chagas Disease with Hypersensitivity Reactions to Benznidazole.

Applications

Unspecified application

Species

Unspecified reactive species

Melisa D Castro Eiro,María A Natale,María G Alvarez,Araceli Castro,Débora Seigelshifer,Rodolfo Viotti,Marisa Fernández,Luis Mazzuoccolo,Bruno Lococo,Graciela L Bertocchi,Gonzalo Cesar,María C Albareda,María J Elias,María B Caputo,Eduardo Gaddi,Jeanette Balbaryski,Carlos A Vigliano,Susana A Laucella

Frontiers in physiology 12:708824 PubMed34366896

2021

Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Ayala-Ramírez,Catalina Machuca-Acevedo,Tatiana Gámez,Sandra Quijano,Alfonso Barreto,Jaime L Silva,Mercedes Olaya-C,Reggie García-Robles

Journal of extracellular vesicles 10:e12022 PubMed33708355

2020

Microglial derived extracellular vesicles activate autophagy and mediate multi-target signaling to maintain cellular homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Bram Van den Broek,Isabel Pintelon,Ibrahim Hamad,Sofie Kessels,Mansour Haidar,Niels Hellings,Jerome J A Hendriks,Markus Kleinewietfeld,Bert Brône,Vincent Timmerman,Jean-Pierre Timmermans,Veerle Somers,Luc Michiels,Joy Irobi

Frontiers in pediatrics 6:139 PubMed29977885

2018

Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Xinlun Dai,Ji Qi,Yu Ao,Chunfeng Yang,Yumei Li
View all publications
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