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AB45892

Human FAS Ligand ELISA Kit (CD95L)

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(1 Publication)

Human FAS Ligand ELISA Kit (CD95L) is a Sandwich (quantitative) ELISA for the measurement of Human FAS Ligand (CD95L) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL

1 Images
Sandwich ELISA - Human FAS Ligand ELISA Kit (CD95L) (AB45892)
  • sELISA

Supplier Data

Sandwich ELISA - Human FAS Ligand ELISA Kit (CD95L) (AB45892)

Representative standard curve using ab45892.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 12 pg/mL

Range

62.5 - 2000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human FAS Ligand in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FAS Ligand in Human serum, plasma, buffered solutions and cell culture media.

A monoclonal antibody specific for FAS Ligand has been coated onto the wells of the microtiter strips provided. Samples, including standards of known FAS Ligand concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for FAS Ligand are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of FAS Ligand present in the samples.

This kit will recognize both endogenous and recombinant Human FAS Ligand.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 35, "mean": "302.2 pg/mL", "standardDeviation": "14.6", "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 40, "mean": "588.4 pg/mL", "standardDeviation": "22.6", "coefficientOfVariability": "3.8" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab45892-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB45892-CMP06" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB45892-CMP02" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB45892-CMP08" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45892-CMP07" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-FAS Ligand", "number":"AB45892-CMP04" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB45892-CMP10" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB45892-CMP05" }, { "size":"2 x 1 Unit", "name":"FAS Ligand Microplate (12 x 8 well strips)", "number":"AB45892-CMP03" }, { "size":"4 x 1 Vial", "name":"FAS Ligand Standard (Lyophilized)", "number":"AB45892-CMP09" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45892-CMP01" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45892-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab45892-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB45892-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB45892-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"FAS Ligand Standard (Lyophilized)", "number":"AB45892-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB45892-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"FAS Ligand Microplate (12 x 8 well strips)", "number":"AB45892-CMP03", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45892-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45892-CMP11", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45892-CMP07", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-FAS Ligand", "number":"AB45892-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB45892-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB45892-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed : 26334989, PubMed : 9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed : 7528780, PubMed : 9228058, PubMed : 9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed : 27806260).. Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed : 27806260).. FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
See full target information FASLG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of surgical research 218:180-193 PubMed28985848

2017

Transforming growth factor β1 and Fas ligand synergistically enhance immune tolerance in dendritic cells in liver transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Minglian Qiu,Yujuan Chen,Lihong Chen,Jinhua Zeng,Jingfeng Liu
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websiteProtocolBooklet
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