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AB118182

Human Fatty Acid Oxidation In-Cell ELISA Kit

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(4 Publications)

Human Fatty Acid Oxidation In-Cell ELISA Kit is a Cell-based ELISA for the measurement of Human Fatty Acid Oxidation In-Cell in in Cell Samples samples.

View Alternative Names

VLCAD, ACADVL

4 Images
Immunocytochemistry - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182)
  • ICC

Supplier Data

Immunocytochemistry - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182)

Antibody specificity demonstrated by immunocytochemistry. Visualization under the microscope was carried out with a completely opened aperture and a very narrow field of visualization at 40x. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab. Note that although LCHAD#1 deficient had a characterized homozygous mutation, the antibody shows a mosaic pattern of staining with about 1/3 of the cells lacking HADHA staining and an overall 40% reduction of signal as observed by ICE.

In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182)
  • In-Cell ELISA

Supplier Data

In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182)

Panel B shows levels of ACADM protein in two deficient cell lines (ACADM : p. [K304E]).

In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182)
  • In-Cell ELISA

Supplier Data

In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182)

Panel A shows levels of ACADVL protein in a deficient cell line (ACADVL : p.[N122D]).

In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182)
  • In-Cell ELISA

Supplier Data

In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182)

Panel C shows levels of HADHA protein in two deficient cell lines : (1) HADHA : p.[E474Q] and (2) HADHB : p. [R61H];[R247H]. Note that although LCHAD#1 deficient has a partial deficiency as observed by ICC.

Key facts

Detection method

Colorimetric

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.

Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought separately.

Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab118182-1x96Tests", "assetComponentDetails": [ { "size":"1 x 0.12 mL", "name":"100X ACADM Primary Antibody", "number":"AB118182-CMP02", "productcode":"" }, { "size":"1 x 0.04 mL", "name":"1000X IRDye 800-labeled Secondary antibody", "number":"AB118182-CMP07", "productcode":"" }, { "size":"1 x 0.12 mL", "name":"100X HADHA Primary Antibody", "number":"AB118182-CMP06", "productcode":"" }, { "size":"1 x 4 mL", "name":"400X Tween-20", "number":"AB118182-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"33x Triton X-100", "number":"AB118182-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Blocking Buffer", "number":"AB118182-CMP08", "productcode":"" }, { "size":"1 x 100 mL", "name":"10X Phosphate Buffered Saline", "number":"AB118182-CMP01", "productcode":"" }, { "size":"1 x 17 mL", "name":"1X Janus Green Stain", "number":"AB118182-CMP09", "productcode":"" }, { "size":"1 x 0.12 mL", "name":"100X ACADVL Primary Antibody", "number":"AB118182-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 17564966, PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9599005, PubMed : 9839948). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on fatty acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed : 17564966, PubMed : 21237683, PubMed : 9839948).
See full target information ACADVL
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:627-647 PubMed38206305

2024

Targeting of FSP1 regulates iron homeostasis in drug-tolerant persister head and neck cancer cells via lipid-metabolism-driven ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang-Che Wu,Chin-Sheng Huang,Ming-Shou Hsieh,Chih-Ming Huang,Syahru Agung Setiawan,Chi-Tai Yeh,Kuang-Tai Kuo,Shao-Cheng Liu

Oxidative medicine and cellular longevity 2022:5265616 PubMed36035217

2022

Contribution of Adiponectin/Carnitine Palmityl Transferase 1A-Mediated Fatty Acid Metabolism during the Development of Idiopathic Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Wu,Guojun Zhang,Lingxiao Qiu,Xueya Liu,Shuai Zhou,Jizhen Wu

Life science alliance 3: PubMed32487689

2020

Snail augments fatty acid oxidation by suppression of mitochondrial ACC2 during cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hye Yang,Nam Hee Kim,Jun Seop Yun,Eunae Sandra Cho,Yong Hoon Cha,Sue Bean Cho,Seon-Hyeong Lee,So Young Cha,Soo-Youl Kim,Jiwon Choi,Tin-Tin Manh Nguyen,Sunghyouk Park,Hyun Sil Kim,Jong In Yook

Journal of biomedical science 25:27 PubMed29592806

2018

β-aminoisobutyric acid attenuates LPS-induced inflammation and insulin resistance in adipocytes through AMPK-mediated pathway.

Applications

FuncS

Species

Unspecified reactive species

Tae Woo Jung,Hyung Sub Park,Geum Hee Choi,Daehwan Kim,Taeseung Lee
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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