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Human Fatty Acid Oxidation In-Cell ELISA Kit is Cell-based (quantitative) ELISA for the measurement of Human Fatty Acid Oxidation production in in Suspension cells, Adherent cells samples.

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Images

In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182), expandable thumbnail
  • In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182), expandable thumbnail
  • Immunocytochemistry - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182), expandable thumbnail
  • In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (AB118182), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9599005, PubMed:9839948). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:18227065, PubMed:7668252, PubMed:9461620, PubMed:9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed:21237683, PubMed:9839948).

Alternative names

What's included?

1 x 96 Tests
Components
1000X IRDye 800-labeled Secondary antibody
1 x 0.04 mL
100X ACADM Primary Antibody
1 x 0.12 mL
100X ACADVL Primary Antibody
1 x 0.12 mL
100X HADHA Primary Antibody
1 x 0.12 mL
10X Blocking Buffer
1 x 20 mL
10X Phosphate Buffered Saline
1 x 100 mL
1X Janus Green Stain
1 x 17 mL
33x Triton X-100
1 x 5 mL
400X Tween-20
1 x 4 mL

Recommended products

Human Fatty Acid Oxidation In-Cell ELISA Kit is Cell-based (quantitative) ELISA for the measurement of Human Fatty Acid Oxidation production in in Suspension cells, Adherent cells samples.

Key facts

Detection method

Colorimetric

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Microplate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.

Plates are available in our ICE (In-Cell ELISA) Support Pack (In-Cell ELISA (ICE) Support Pack ab111542) which can be bought separately.

Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182), expandable thumbnail

    In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182)

    Panel A shows levels of ACADVL protein in a deficient cell line (ACADVL:p.[N122D]).

  • In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182), expandable thumbnail

    In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182)

    Panel C shows levels of HADHA protein in two deficient cell lines: (1) HADHA:p.[E474Q] and (2) HADHB:p. [R61H];[R247H]. Note that although LCHAD#1 deficient has a partial deficiency as observed by ICC.

  • Immunocytochemistry - Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182), expandable thumbnail

    Immunocytochemistry - Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182)

    Antibody specificity demonstrated by immunocytochemistry. Visualization under the microscope was carried out with a completely opened aperture and a very narrow field of visualization at 40x. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab. Note that although LCHAD#1 deficient had a characterized homozygous mutation, the antibody shows a mosaic pattern of staining with about 1/3 of the cells lacking HADHA staining and an overall 40% reduction of signal as observed by ICE.

  • In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182), expandable thumbnail

    In-Cell ELISA - Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182)

    Panel B shows levels of ACADM protein in two deficient cell lines (ACADM:p. [K304E]).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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