Human Fatty Acid Synthase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Fatty Acid Synthase with a sensitivity of 3.3 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Journal of translational medicine 22:552024Metabolic reprogramming based on RNA sequencing of gemcitabine-resistant cells reveals the FASN gene as a therapeutic for bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijie Zhou,Kaixuan Du,Yiheng Dai,Youmiao Zeng,Yongbo Luo,Mengda Ren,Wenbang Pan,Yuanhao Liu,Lailai Zhang,Ronghui Zhu,Dapeng Feng,Fengyan Tian,Chaohui GuPubMed 38218866
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 10.9 - 700 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.3 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Alternative names
FAS, FASN, Fatty acid synthase, Type I fatty acid synthase
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Recommended products
Human Fatty Acid Synthase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Fatty Acid Synthase with a sensitivity of 3.3 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 10.9 - 700 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.3 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 4.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 4 | mean - | SD - | C.V. 5.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 111 | Range 102 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Fatty Acid Synthase ELISA kit (ab279412) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Fatty Acid Synthase protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fatty Acid Synthase with 3.3 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Fatty acid synthase, also known as Type I fatty acid synthase, is an multienzyme encoded by the FASN gene. FASN contains two identical subunits in a head-to-tail connection of a 272 kDa monomer, each containing seven known catalytic activities, including ketoacyl synthase, malonyl/acetyltransferase, dehydrase, enoyl reductase, ketoacyl reductase, acyl carrier protein, and thioesterase. Fatty acid synthase catalyzes the biosynthesis of long-chain saturated fatty acids, particularly C16 palmitate, from acetyl-CoA and malonyl-CoA in the presence of NADPH.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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2 product images
Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (ab279412)
Interpolated concentrations of native Fatty Acid Synthase in human HEK293T cell extract and based on a 25 μg/mL extract load.
The concentrations of Fatty Acid Synthase were measured in duplicate and interpolated from the Fatty Acid Synthase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (ab279412)
The Fatty Acid Synthase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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