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AB279412

Human Fatty Acid Synthase ELISA Kit

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(1 Publication)

Human Fatty Acid Synthase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Fatty Acid Synthase with a sensitivity of 3.3 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

FAS, FASN, Fatty acid synthase, Type I fatty acid synthase

3 Images
Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (AB279412)
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Supplier Data

Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (AB279412)

Interpolated concentrations of native Fatty Acid Synthase in human HEK293T cell extract and based on a 25 μg/mL extract load.

The concentrations of Fatty Acid Synthase were measured in duplicate and interpolated from the Fatty Acid Synthase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (AB279412)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (AB279412)

Representative image of Human Fatty Acid Synthase ELISA Kit (ab279412)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Fatty Acid Synthase Capture Antibody

- 10X Human Fatty Acid Synthase Detector Antibody

- Human Fatty Acid Synthase Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (AB279412)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (AB279412)

The Fatty Acid Synthase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.3 ng/mL

Range

10.9 - 700 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Fatty Acid Synthase ELISA kit (ab279412) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Fatty Acid Synthase protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fatty Acid Synthase with 3.3 ng/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Fatty acid synthase, also known as Type I fatty acid synthase, is an multienzyme encoded by the FASN gene. FASN contains two identical subunits in a head-to-tail connection of a 272 kDa monomer, each containing seven known catalytic activities, including ketoacyl synthase, malonyl/acetyltransferase, dehydrase, enoyl reductase, ketoacyl reductase, acyl carrier protein, and thioesterase. Fatty acid synthase catalyzes the biosynthesis of long-chain saturated fatty acids, particularly C16 palmitate, from acetyl-CoA and malonyl-CoA in the presence of NADPH.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "102 - 117 %", "average": "= 111" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279412-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB279412-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB279412-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB279412-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB279412-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB279412-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB279412-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB279412-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Fatty Acid Synthase Capture Antibody", "number":"AB279412-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB279412-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Fatty Acid Synthase Lyophilized Recombinant Protein", "number":"AB279412-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Fatty Acid Synthase Detector Antibody", "number":"AB279412-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB279412-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
See full target information FASN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:55 PubMed38218866

2024

Metabolic reprogramming based on RNA sequencing of gemcitabine-resistant cells reveals the FASN gene as a therapeutic for bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Zhou,Kaixuan Du,Yiheng Dai,Youmiao Zeng,Yongbo Luo,Mengda Ren,Wenbang Pan,Yuanhao Liu,Lailai Zhang,Ronghui Zhu,Dapeng Feng,Fengyan Tian,Chaohui Gu
View all publications
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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