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AB316890

Human Fc epsilon RI/FCER1A ELISA Kit

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Human Fc epsilon RI/FCER1A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Fc epsilon RI/FCER1A with a sensitivity of 5.22 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

FCE1A, FCER1A, High affinity immunoglobulin epsilon receptor subunit alpha, Fc-epsilon RI-alpha, IgE Fc receptor subunit alpha, FcERI

2 Images
Sandwich ELISA - Human Fc epsilon RI/FCER1A ELISA Kit (AB316890)
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Supplier Data

Sandwich ELISA - Human Fc epsilon RI/FCER1A ELISA Kit (AB316890)

Interpolated concentrations of human Fc epsilon R1/FCER1A in Jurkat and HEL92.1.7 cell extracts

Interpolated concentration of native Fc epsilon R1/FCER1A was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Jurkat cell extract 50 µg/mL, HEL92.1.7 cell extract 12.5 µg/mL.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR. No signal was detected in Jurkat cell extract.

Sandwich ELISA - Human Fc epsilon RI/FCER1A ELISA Kit (AB316890)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fc epsilon RI/FCER1A ELISA Kit (AB316890)

Example of human Fc epsilon R1/FCER1A standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.22 pg/mL

Range

17.188 - 1100 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB316199

Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fc epsilon RI/FCER1A antibody [EPR28402-78] (AB316199)

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Proteins & Peptides

AB171505

Recombinant Human Fc epsilon RI/FCER1A protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Fc epsilon RI/FCER1A protein (His tag N-Terminus) (AB171505)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Fc epsilon RI/FCER1A SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fc epsilon RI/FCER1A protein in Cell Lysate. Quantitate Human Fc epsilon RI/FCER1A with 5.22 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.3" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "97 - 114 %", "average": "= 107" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

High-affinity receptor for immunoglobulin epsilon/IgE. Mediates IgE effector functions in myeloid cells. Upon IgE binding and antigen/allergen cross-linking initiates signaling pathways that lead to myeloid cell activation and differentiation. On mast cells, basophils and eosinophils stimulates the secretion of vasoactive amines, lipid mediators and cytokines that contribute to inflammatory response, tissue remodeling and cytotoxicity against microbes. Triggers the immediate hypersensitivity response to allergens as a host defense mechanism against helminth parasites, pathogenic bacteria and venom toxicity. When dysregulated, it can elicit harmful life-threatening allergic and anaphylactic reactions.
See full target information FCER1A
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com