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AB213777

Human FCN1/M-Ficolin ELISA Kit

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(2 Publications)

Human FCN1/M-Ficolin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human FCN1/M-Ficolin in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

FCNM, FCN1, Ficolin-1, Collagen/fibrinogen domain-containing protein 1, Ficolin-A, Ficolin-alpha, M-ficolin

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Sandwich ELISA - Human FCN1/M-Ficolin ELISA Kit (AB213777)
  • sELISA

Supplier Data

Sandwich ELISA - Human FCN1/M-Ficolin ELISA Kit (AB213777)

Human FCN1/M-Ficolin ELISA Kit (ab213777) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

0.78 - 50 ng/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human FCN1/M-Ficolin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213777) is designed for the quantitative measurement of Human FCN1/M-Ficolin in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FCN1/M-Ficolin has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A30-A326) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FCN1/M-Ficolin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FCN1/M-Ficolin amount of sample captured in plate.

FCN1/M-Ficolin, also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. FCN1/M-Ficolin is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "8.931 ng/mL", "standardDeviation": "0.544", "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "8.333 ng/mL", "standardDeviation": "0.483", "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "19.839 ng/mL", "standardDeviation": "1.091", "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1.265 ng/mL", "standardDeviation": "0.095", "coefficientOfVariability": "= 7" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "19.299 ng/mL", "standardDeviation": "1.042", "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1.355 ng/mL", "standardDeviation": "0.088", "coefficientOfVariability": "= 6.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213777-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213777-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human FCN1/M-Ficolin antibody", "number":"AB213777-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human FCN1/M-Ficolin coated Microplate (12 x 8 wells)", "number":"AB213777-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213777-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213777-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213777-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213777-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213777-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human FCN1/M-Ficolin standard", "number":"AB213777-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213777-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213777-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FCN1 also known as M-Ficolin or ficolin-1 is a pattern recognition molecule part of the innate immune system. It plays a role in recognizing pathogen-associated molecular patterns (PAMPs) on the surface of microbes. FCN1 is a 35 kDa protein predominantly expressed in the liver and secreted into the bloodstream. It consists of a collagen-like domain and a fibrinogen-like recognition domain allowing it to effectively bind its targets. The protein occurs in plasma where it particularly acts in host defense mechanisms.
Biological function summary

FCN1 functions as part of the body’s first line of immune defense by binding to microbial surfaces facilitating the opsonization and activation of the lectin complement pathway. It is a member of a complex known as the lectin pathway of complement activation which includes MASP1 and MASP2 proteins. FCN1 triggers this pathway when it recognizes patterns such as N-acetylglucosamine on the surface of pathogens which is important for initiating the immune response and promoting pathogen clearance through complement activation in cooperation with other components of the pathway.

Pathways

FCN1 primarily participates in the lectin complement pathway distinctively different from the classical and alternative pathways. It interacts with MASP proteins to trigger the cleavage of complement proteins which leads to a cascade that results in inflammation opsonization and pathogen lysis. FCN1 connections also extend to other pattern recognition receptors such as those in the toll-like receptor pathway which harmonize to ensure a robust immune response by recognizing wide array of pathogen motifs.

FCN1 involvement has been observed in several conditions notably systemic lupus erythematosus (SLE) and bacterial infections. Alterations or deficiencies in FCN1 levels can influence susceptibility to infections due to compromised opsonization and pathogen clearance. In SLE FCN1 and its associated pathway proteins may contribute to the disease pathogenesis by affecting complement system activity leading to autoimmune and inflammatory responses. Understanding these associations aids in unraveling the complex network of immune response regulation and disease development.
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Product protocols

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Target data

Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8 (PubMed : 21037097). Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage.
See full target information FCN1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 8: PubMed38829196

2024

Proteomic analysis of serum extracellular vesicles reveals Fibulin-3 as a new marker predicting liver-related events in MASLD.

Applications

Unspecified application

Species

Unspecified reactive species

Sadatsugu Sakane,Hayato Hikita,Kumiko Shirai,Tatsuya Sakamoto,Ryohei Narumi,Jun Adachi,Naruyasu Kakita,Yukinori Yamada,Hidenori Toyoda,Hirokazu Takahashi,Goki Suda,Machiko Kai,Yuki Tahata,Ryotaro Sakamori,Shusuke Kumazaki,Kenji Fukumoto,Yuta Myojin,Kazuhiro Murai,Takahiro Kodama,Tomohide Tatsumi,Takeshi Tomonaga,Naoya Sakamoto,Eiichi Morii,Tetsuo Takehara

Cell death & disease 14:577 PubMed37648705

2023

Ficolin B secreted by alveolar macrophage exosomes exacerbates bleomycin-induced lung injury via ferroptosis through the cGAS-STING signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Wu,Yixia Jiang,Rong Li,Yezhou Xia,Feifan Li,Meiyun Zhao,Guoqing Li,Xiaowu Tan
View all publications
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