Human FCRL2 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human FCRL2 with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 34.375 - 2200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May have an regulatory role in normal and neoplastic B cell development.
Alternative names
CD307b, FCRH2, IFGP4, IRTA4, SPAP1, UNQ9236/PRO31998, FCRL2, Fc receptor-like protein 2, FcR-like protein 2, FcRL2, Fc receptor homolog 2, IFGP family protein 4, Immunoglobulin receptor translocation-associated protein 4, SH2 domain-containing phosphatase anchor protein 1, FcRH2
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Human FCRL2 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human FCRL2 with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 34.375 - 2200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3 pg/mL
- General recovery
- 86%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 5.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 4.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 86 | Range 82 - 91 % |
Sample type EDTA Plasma | Average % = 89 | Range 82 - 95 % |
Sample type Citrate plasma | Average % = 86 | Range 82 - 92 % |
Sample type Heparin Plasma | Average % = 86 | Range 78 - 91 % |
Sample type Cell culture supernatant | Average % = 102 | Range 97 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human FCRL2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FCRL2 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human FCRL2 with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FCRL2 also known as FC Receptor-like 2 or CD307b belongs to the family of Fc receptor-like molecules. It is a transmembrane receptor with a mass of approximately 110 kDa. FCRL2 shows expression mainly in B cells with higher levels notably in some mature B cell subtypes found in the lymphoid tissues. Its expression is significantly observed in the follicles of the spleen and tonsils indicating a role in the immune responses centered around these tissues.
- Biological function summary
FCRL2 modulates signaling in B cells partaking in immune regulation. It participates in the regulatory networks that adjust B-cell function and differentiation but it does not form any known complex with other proteins. The protein has a structural similarity to classical Fc receptors that suggests a modulatory role in immune response. Researchers consider FCRL2 significant in altering B cell receptor signaling though the exact ligands remain under active investigation.
- Pathways
The protein FCRL2 integrates into immune signaling pathways such as the B cell receptor (BCR) signaling pathway. This pathway plays a direct role in B cell activation and antibody production. FCRL2 interacts with other FCRLS members in such pathways to maintain immune homeostasis. The extent of its integration implies a fine-tuning of B cell functions and links to Fcγ receptors indicating a shared regulatory network in pathways.
- Associated diseases and disorders
FCRL2 associates with conditions like chronic lymphocytic leukemia (CLL) and autoimmune diseases. Increased expression of FCRL2 often correlates with certain leukemia subtypes hinting at its potential as a biomarker for disease progression. Moreover its dysregulation may contribute to autoimmune conditions where B cell dysfunctions are central. Studies have also connected FCRL2 with other immune-regulatory proteins in these diseases further highlighting its relevance in pathological conditions related to immune response dysregulation.
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3 product images
Sandwich ELISA - Human FCRL2 ELISA Kit- extracellular (ab315303)
Interpolated concentration of native FCRL2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 50%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and Daudi cell supernatant 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human FCRL2 ELISA Kit- extracellular (ab315303)
Example of human FCRL2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human FCRL2 ELISA Kit- extracellular (ab315303)
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean FCRL2 concentration was determined to be 443 pg/mL with a range of 193 – 937 pg/mL.
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