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AB315302

Human FCRL2 ELISA Kit- intracellular

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Human FCRL2 ELISA Kit- intracellular is a single-wash 90-min Simplestep used to quantify Human FCRL2 with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD307b, FCRH2, IFGP4, IRTA4, SPAP1, UNQ9236/PRO31998, FCRL2, Fc receptor-like protein 2, FcR-like protein 2, FcRL2, Fc receptor homolog 2, IFGP family protein 4, Immunoglobulin receptor translocation-associated protein 4, SH2 domain-containing phosphatase anchor protein 1, FcRH2

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Sandwich ELISA - Human FCRL2 ELISA Kit- intracellular (AB315302)
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Supplier Data

Sandwich ELISA - Human FCRL2 ELISA Kit- intracellular (AB315302)

Interpolated concentrations of human FCRL2 in Daudi cell extract, Raji cell extract, and spleen tissue extract

Interpolated concentration of native FCRL2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Daudi cell extract 500 µg/mL, Raji cell extract 1,000 µg/mL, and spleen tissue extract 1,000 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human FCRL2 ELISA Kit- intracellular (AB315302)
  • sELISA

Supplier Data

Sandwich ELISA - Human FCRL2 ELISA Kit- intracellular (AB315302)

Example of human FCRL2 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB197897

Anti-FCRL2 antibody [EPR16122]

RabMAb|Recombinant

Immunoprecipitation - Anti-FCRL2 antibody [EPR16122] (AB197897)

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Proteins & Peptides

AB276705

Recombinant Human FCRL2 protein (His tag)

SDS-PAGE - Recombinant Human FCRL2 protein (His tag) (AB276705)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FCRL2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FCRL2 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human FCRL2 with 11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "109 - 120 %", "average": "= 113" }, { "sample": "Tissue Extracts", "range": "109 - 120 %", "average": "= 113" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FCRL2 also known as FC Receptor-like 2 or CD307b belongs to the family of Fc receptor-like molecules. It is a transmembrane receptor with a mass of approximately 110 kDa. FCRL2 shows expression mainly in B cells with higher levels notably in some mature B cell subtypes found in the lymphoid tissues. Its expression is significantly observed in the follicles of the spleen and tonsils indicating a role in the immune responses centered around these tissues.
Biological function summary

FCRL2 modulates signaling in B cells partaking in immune regulation. It participates in the regulatory networks that adjust B-cell function and differentiation but it does not form any known complex with other proteins. The protein has a structural similarity to classical Fc receptors that suggests a modulatory role in immune response. Researchers consider FCRL2 significant in altering B cell receptor signaling though the exact ligands remain under active investigation.

Pathways

The protein FCRL2 integrates into immune signaling pathways such as the B cell receptor (BCR) signaling pathway. This pathway plays a direct role in B cell activation and antibody production. FCRL2 interacts with other FCRLS members in such pathways to maintain immune homeostasis. The extent of its integration implies a fine-tuning of B cell functions and links to Fcγ receptors indicating a shared regulatory network in pathways.

FCRL2 associates with conditions like chronic lymphocytic leukemia (CLL) and autoimmune diseases. Increased expression of FCRL2 often correlates with certain leukemia subtypes hinting at its potential as a biomarker for disease progression. Moreover its dysregulation may contribute to autoimmune conditions where B cell dysfunctions are central. Studies have also connected FCRL2 with other immune-regulatory proteins in these diseases further highlighting its relevance in pathological conditions related to immune response dysregulation.
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Product protocols

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Target data

May have an regulatory role in normal and neoplastic B cell development.
See full target information FCRL2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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