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AB313602

Human FCRL3 ELISA Kit

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Human FCRL3 ELISA Kit is a Sandwich ELISA for the measurement of Human FCRL3 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD307c, FCRH3, IFGP3, IRTA3, SPAP2, FCRL3, Fc receptor-like protein 3, FcR-like protein 3, FcRL3, Fc receptor homolog 3, IFGP family protein 3, Immune receptor translocation-associated protein 3, MAIA, SH2 domain-containing phosphatase anchor protein 2, FcRH3, hIFGP3

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Sandwich ELISA - Human FCRL3 ELISA Kit (AB313602)
  • sELISA

Supplier Data

Sandwich ELISA - Human FCRL3 ELISA Kit (AB313602)

Example of standard curve using ab313602. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.05 ng/mL

Range

0.05 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Proteins & Peptides

AB165072

Recombinant Human FCRL3 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human FCRL3 protein (GST tag N-Terminus) (AB165072)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FCRL3 (FcRH3) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FCRL3 (FcRH3) in serum (human FCRL3 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended in this assay), and cell culture supernatants. This assay employs an antibody specific for human FCRL3 (FcRH3) coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL7, CD36, CLEC9a, CL-P1, Dectin-2, DLL4, DSCAM, EDIL3, ENPP-7, Enteropeptidase, FCRLB, FGF-3, FLRT1, FLRT2, GBA3, GDF-11, Glycoprotein V, Granzyme A, IGSF4B, IL-28 R alpha, Kynureninase, LAMA4, LRRC4, LRRTM3, NG2, NQO-1, PCSK2, PILR-alpha, Plexin A4, POGLUT1, PRELP, Smad4, SOX15, SOX7, SOX9, Syntaxin 6, TROP2, TSLP R, UNC5H4.

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Recovery

[ { "sample": "Serum", "range": "69 - 94 %", "average": "= 80.66" }, { "sample": "Plasma", "range": "68 - 82 %", "average": "= 73.82" }, { "sample": "Cell culture media", "range": "70 - 138 %", "average": "= 90.22" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FCRL3 also known as Fc receptor-like 3 is a protein encoded by the FCRL3 gene. It weighs approximately 75 kDa and plays an important role in immune regulation. FCRL3 is predominantly expressed on B cells but it is also found on T cells and natural killer cells. The protein is part of the family of Fc receptor homologs and features immunoreceptor tyrosine-based activation motifs (ITAMs) and immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These motifs allow FCRL3 to function both as an activator and inhibitor of immune responses depending on the context.
Biological function summary

FCRL3 acts in regulating immune cell activation and differentiation. It influences the balance of auto-reactive and protective immune responses. FCRL3 does not form multimeric complexes but interacts with other immune receptors and signaling proteins. By modulating signaling pathways it impacts processes such as antibody production cellular proliferation and cytokine release. The protein contributes to maintaining immune homeostasis and preventing excessive inflammatory responses.

Pathways

FCRL3 engages in immune signaling cascades notably the B cell receptor (BCR) pathway and the T cell receptor (TCR) pathway. These pathways are central to adaptive immunity involving receptor signaling and subsequent cell activation. FCRL3 interacts with other proteins such as SYK and SHP-1 which mediate downstream signaling events in these pathways. This modulation is important for the fine-tuning of adaptive immune responses influencing immunity against pathogens and tolerance towards self-antigens.

FCRL3 has been associated with autoimmune conditions like rheumatoid arthritis and systemic lupus erythematosus. In these disorders FCRL3 expression influences pathogenic B cell activity and autoantibody production. The protein shares interactions with CD20 and BLIMP-1 both key regulators of B cell function which link alterations in FCRL3 activity with disease progression and severity. Understanding FCRL3's role in these conditions aids in developing targeted therapies for autoimmune diseases.
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Product protocols

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Target data

Promotes TLR9-induced B-cell proliferation, activation and survival but inhibits antibody production and suppresses plasma cell differentiation. Enhances activation of NF-kappa-B and MAPK signaling pathways in TLR9 stimulated B-cells (PubMed : 23857366). Has inhibitory potentional on B-cell receptor (BCR)-mediated signaling, possibly through association with SH2 domain-containing phosphatases. Inhibits cell tyrosine phosphorylation, calcium mobilization and activation-induced cell death induced through BCR signaling (PubMed : 19843936). Regulatory T-cells expressing FCRL3 exhibit a memory phenotype, are relatively nonresponsive to antigenic stimulation in presence of IL2 and have reduced capacity to suppress the proliferation of effector T-cells (PubMed : 19494275, PubMed : 20190142). Acts as a human-specific epitope on the cell surface of oocytes (oolemma) and plays a role during sperm-egg adhesion and fusion (PubMed : 36070373). Interacts with the IZUMO1-IZUMO1R/JUNO sperm-egg complex and replaces IZUMO1R/JUNO as IZUMO1 receptor during fertilization, thereby permitting species-specific gamete fusion (PubMed : 36070373).
See full target information FCRL3
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Product promise

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