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Human FDPS/FPS ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human FDPS/FPS in Human in Plasma, Serum, Cell culture supernatant samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1.62 - 400 ng/mL

Sensitivity

= 1.62 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

Alternative names

What's included?

1 x 96 Tests
Components
200X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
96-Well Plate Sealing Film
1 x 1 Unit
Biotinylated Human FDPS Detection Antibody
2 x 1 Vial
Human FDPS Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human FDPS Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human FDPS/FPS ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human FDPS/FPS in Human in Plasma, Serum, Cell culture supernatant samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1.62 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 1.62 ng/mL

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 107.9

Range

95 - 118 %

Sample type

Plasma

Average %

= 96.57

Range

72 - 119 %

Sample type

Cell culture supernatant

Average %

= 134.6

Range

124 - 140 %

Sample type

Cell culture media

Average %

= 134.6

Range

124 - 140 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human FDPS/FPS ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human FDPS/FPS coated on a 96-well plate.

Supplementary info

Biological function summary

Farnesyl diphosphate synthase facilitates the production of FPP a common precursor for the synthesis of isoprenoid compounds such as cholesterol ubiquinones and dolichol. FDPS does not form a complex but works in coordination with other enzymes in the mevalonate pathway. Its activity supports numerous cellular functions including membrane assembly protein prenylation and cell signaling. The enzyme’s role is important for normal cellular growth and development influencing processes like cell differentiation and proliferation.

Activity summary

Farnesyl diphosphate synthase (FDPS) also known as farnesyl pyrophosphate synthase (FPS) is an important enzyme that catalyzes the synthesis of farnesyl diphosphate (FPP) from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). This enzyme plays an important role in the mevalonate pathway which is essential for the biosynthesis of sterols including cholesterol and other isoprenoids. FDPS is a protein with a molecular mass of approximately 42 kDa. Its expression occurs in various tissues with significant levels in liver brain and bone where it contributes to different cellular metabolic processes.

Pathways

FDPS plays a significant role in the cholesterol biosynthesis and protein prenylation pathways. These pathways are critical for cellular homeostasis and involve several enzymes including HMG-CoA reductase and squalene synthase. The cholesterol biosynthesis pathway includes multiple steps where FPP serves as a lipid intermediate. Meanwhile in protein prenylation FPP contributes to the post-translational modification of proteins such as small GTPases which impacts cell signaling and cytoskeletal organization.

Associated diseases and disorders

FDPS has a connection to bone diseases such as osteoporosis and also links to cardiovascular disorders. The relationship to osteoporosis arises from its role in bone resorption and osteoclast activity mediated by proteins like RANKL and osteoprotegerin. Cardiovascular disorders may link to altered cholesterol metabolism with FDPS influencing cholesterol biosynthesis and regulation. Inhibition of FDPS activity targets these pathways leading to therapeutic strategies that may address both metabolic and bone health issues.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human FDPS/FPS ELISA Kit (ab309408), expandable thumbnail

    Sandwich ELISA - Human FDPS/FPS ELISA Kit (ab309408)

    Example of standard curve using ab309408. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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