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AB309408

Human FDPS/FPS ELISA Kit

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Human FDPS/FPS ELISA Kit is a Sandwich ELISA for the measurement of Human FDPS/FPS in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

FPS, KIAA1293, FDPS, Farnesyl pyrophosphate synthase, FPP synthase, Dimethylallyltranstransferase, Farnesyl diphosphate synthase, Geranyltranstransferase

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Sandwich ELISA - Human FDPS/FPS ELISA Kit (AB309408)
  • sELISA

Supplier Data

Sandwich ELISA - Human FDPS/FPS ELISA Kit (AB309408)

Example of standard curve using ab309408. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.62 ng/mL

Range

1.62 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB153805

Anti-FDPS/FPS antibody

Western blot - Anti-FDPS/FPS antibody (AB153805)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human FDPS/FPS ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human FDPS/FPS coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "95 - 118 %", "average": "= 107.9" }, { "sample": "Plasma", "range": "72 - 119 %", "average": "= 96.57" }, { "sample": "Cell culture supernatant", "range": "124 - 140 %", "average": "= 134.6" }, { "sample": "Cell culture media", "range": "124 - 140 %", "average": "= 134.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309408-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309408-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309408-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309408-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human FDPS Detection Antibody", "number":"AB309408-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309408-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human FDPS Protein Standard", "number":"AB309408-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human FDPS Antibody-coated ELISA Plate", "number":"AB309408-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309408-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB309408-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Farnesyl diphosphate synthase (FDPS) also known as farnesyl pyrophosphate synthase (FPS) is an important enzyme that catalyzes the synthesis of farnesyl diphosphate (FPP) from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). This enzyme plays an important role in the mevalonate pathway which is essential for the biosynthesis of sterols including cholesterol and other isoprenoids. FDPS is a protein with a molecular mass of approximately 42 kDa. Its expression occurs in various tissues with significant levels in liver brain and bone where it contributes to different cellular metabolic processes.
Biological function summary

Farnesyl diphosphate synthase facilitates the production of FPP a common precursor for the synthesis of isoprenoid compounds such as cholesterol ubiquinones and dolichol. FDPS does not form a complex but works in coordination with other enzymes in the mevalonate pathway. Its activity supports numerous cellular functions including membrane assembly protein prenylation and cell signaling. The enzyme’s role is important for normal cellular growth and development influencing processes like cell differentiation and proliferation.

Pathways

FDPS plays a significant role in the cholesterol biosynthesis and protein prenylation pathways. These pathways are critical for cellular homeostasis and involve several enzymes including HMG-CoA reductase and squalene synthase. The cholesterol biosynthesis pathway includes multiple steps where FPP serves as a lipid intermediate. Meanwhile in protein prenylation FPP contributes to the post-translational modification of proteins such as small GTPases which impacts cell signaling and cytoskeletal organization.

FDPS has a connection to bone diseases such as osteoporosis and also links to cardiovascular disorders. The relationship to osteoporosis arises from its role in bone resorption and osteoclast activity mediated by proteins like RANKL and osteoprotegerin. Cardiovascular disorders may link to altered cholesterol metabolism with FDPS influencing cholesterol biosynthesis and regulation. Inhibition of FDPS activity targets these pathways leading to therapeutic strategies that may address both metabolic and bone health issues.
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Product protocols

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Target data

Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.
See full target information FDPS
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websiteProtocolBooklet
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Product promise

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