Human Ferritin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Ferritin in Human in Cerebral Spinal Fluid, Plasma, Milk, Serum samples.
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- Molecules (Basel, Switzerland) 28:2023Edible Halophytes with Functional Properties: In Vitro Protein Digestibility and Bioaccessibility and Intestinal Absorption of Minerals and Trace Elements from Australian Indigenous Halophytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSukirtha Srivarathan,Rama Addepalli,Oladipupo Qudus Adiamo,Gethmini Kavindya Kodagoda,Anh Dao Thi Phan,Olivia Renee Louise Wright,Yasmina Sultanbawa,Simone Osborne,Michael Erich NetzelPubMed 37241743
- Cell death & disease 13:6532022The interaction between STING and NCOA4 exacerbates lethal sepsis by orchestrating ferroptosis and inflammatory responses in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Wu,Qinjie Liu,Xufei Zhang,Miaomiao Tan,Xuanheng Li,Peizhao Liu,Lei Wu,Fan Jiao,Zhaoyu Lin,Xiuwen Wu,Xin Wang,Yun Zhao,Jianan RenPubMed 35902564
- Neuropsychiatric disease and treatment 17:2041-20512021Clinical Characteristics, Iron Metabolism and Neuroinflammation: New Insight into Excessive Daytime Sleepiness in Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Hu,Peng Guo,Teng-Hong Lian,Li-Jun Zuo,Shu-Yang Yu,Li Liu,Zhao Jin,Qiu-Jin Yu,Rui-Dan Wang,Li-Xia Li,Ying-Shan Piao,Wei ZhangPubMed 34188474
- Heliyon 7:e067202021Increased serum ferritin is associated with oxidized low-density lipoprotein in prediabetes patients: A pilot study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Manuel Martínez-Soto,Maria Del Carmen Candia-Plata,Luis Fernando López-Soto,Jesús Adriana Soto-Guzmán,Alma Yolanda Camacho-Villa,Gerardo Álvarez-Hernández,Ana Lourdes Mata-Pineda,José Manuel Galván-MoroyoquiPubMed 33912708
- American journal of respiratory and critical care medicine 200:209-2192019The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah J Allden,Patricia P Ogger,Poonam Ghai,Peter McErlean,Richard Hewitt,Richard Toshner,Simone A Walker,Peter Saunders,Shaun Kingston,Philip L Molyneaux,Toby M Maher,Clare M Lloyd,Adam J ByrnePubMed 31051082
- Frontiers in neuroscience 13:2552019Tremor-Dominant in Parkinson Disease: The Relevance to Iron Metabolism and Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeng-Hong Lian et. al.PubMed 30971879
- Experimental physiology 103:1443-14472018Plasma ferritin concentration is positively associated with in vivo fatty acid mobilization and insulin resistance in obese women.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin J Ryan,Douglas W Van Pelt,Lisa M Guth,Alison C Ludzki,Rachel A Gioscia-Ryan,Chiwoon Ahn,Katherine L Foug,Jeffrey F HorowitzPubMed 30178895
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:2228-22402018Ferroportin disease mutations influence manganese accumulation and cytotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Kyung Choi,Trang-Tiffany Nguyen,Shigeki Iwase,Young Ah SeoPubMed 30247984
- Malaria journal 17:2532018Haemolysis and haem oxygenase-1 induction during persistent "asymptomatic" malaria infection in Burkinabé children.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJason P Mooney et. al.PubMed 29980206
- Journal of neuroinflammation 15:302018The role of HFE genotype in macrophage phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne M Nixon et. al.PubMed 29391061
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Plasma, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.781 - 50 ng/mL
- Assay time
- 4h
- Sensitivity
- = 0.5 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed:24695223, PubMed:26436293).
Additional Targets
FTL
Alternative names
FTH, FTHL6, OK/SW-cl.84, PIG15, FTH1, Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein
What's included?
Recommended products
Human Ferritin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Ferritin in Human in Cerebral Spinal Fluid, Plasma, Milk, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Plasma, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.781 - 50 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 0.5 ng/mL
- General recovery
- 95%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Ferritin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ferritin concentrations in plasma, serum, milk, cerebrospinal fluid samples.
A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product promise
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Full details and terms and conditions can be found here:
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3 product images
Sandwich ELISA - Human Ferritin ELISA Kit (ab108837)
Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample. Serum and plasma samples were diluted 10-20 fold. Milk, urine and PBMC supernatant were diluted 1-4 fold.
Sandwich ELISA - Human Ferritin ELISA Kit (ab108837)
Human Ferritin standard curve: mean of duplicates (+/- SD)
Sandwich ELISA - Human Ferritin ELISA Kit (ab108837)
Representative Standard Curve using ab108837
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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