Human Ferritin Light Chain ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Ferritin Light Chain with a sensitivity of 6.076 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Milk, Saliva, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.076 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).
Alternative names
Ferritin light chain, Ferritin L subunit, FTL
What's included?
Recommended products
Human Ferritin Light Chain ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Ferritin Light Chain with a sensitivity of 6.076 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Milk, Saliva, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6.076 pg/mL
- General recovery
- 98%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 98 | Range 95 - 102 % |
Sample type EDTA Plasma | Average % = 100 | Range 97 - 104 % |
Sample type Citrate plasma | Average % = 94 | Range 88 - 98 % |
Sample type Heparin Plasma | Average % = 112 | Range 108 - 117 % |
Sample type Urine | Average % = 96 | Range 95 - 97 % |
Sample type Milk | Average % = 102 | Range 98 - 106 % |
Sample type Saliva | Average % = 101 | Range 96 - 104 % |
Sample type Cell culture supernatant | Average % = 106 | Range 102 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Ferritin Light Chain SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Ferritin Light Chain protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, Milk, Saliva, Cell culture supernatant. Quantitate Human Ferritin Light Chain with 6.076 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human Ferritin Light Chain ELISA Kit- Extracellular (ab316893)
Example of human Ferritin Light Chain standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Ferritin Light Chain ELISA Kit- Extracellular (ab316893)
Interpolated concentration of native Ferritin Light Chain was measured in duplicate at different sample concentrations. Undiluted samples are as follows: urine 50%, milk 12.5%, saliva 50%, and untreated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Ferritin Light Chain ELISA Kit- Extracellular (ab316893)
Interpolated concentration of native Ferritin Light Chain was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 5%, plasma – citrate 5%, plasma – EDTA 10%, and plasma – heparin 10%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Ferritin Light Chain ELISA Kit- Extracellular (ab316893)
Serum of eight individual healthy human Female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ferritin Light Chain concentration was determined to be 55.6 ng/mL with a range of 2.1 – 210.4 ng/mL.
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