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AB316894

Human Ferritin Light Chain ELISA Kit- Intracellular

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Human Ferritin Light Chain ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Ferritin Light Chain with a sensitivity of 8.589 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Ferritin light chain, Ferritin L subunit, FTL

2 Images
Sandwich ELISA - Human Ferritin Light Chain ELISA Kit- Intracellular (AB316894)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ferritin Light Chain ELISA Kit- Intracellular (AB316894)

Example of human Ferritin Light Chain standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Ferritin Light Chain ELISA Kit- Intracellular (AB316894)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ferritin Light Chain ELISA Kit- Intracellular (AB316894)

Interpolated concentrations of human Ferritin Light Chain in HeLa cell extract, HepG2 cell extract, and human liver extract.

Interpolated concentration of native Ferritin Light Chain was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 31.25 µg/mL HeLa cell extract, 31.25 µg/mL HepG2 cell extract, and 0.12 µg/mL human liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.589 pg/mL

Range

29.688 - 1900 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Ferritin Light Chain SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Ferritin Light Chain protein in Cell Lysate, Tissue extracts. Quantitate Human Ferritin Light Chain with 8.589 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.1" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "96 - 110 %", "average": "= 101" }, { "sample": "Tissue Extracts", "range": "97 - 102 %", "average": "= 99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316894-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB316894-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316894-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316894-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316894-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316894-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB316894-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Ferritin Light Chain Lyophilized Recombinant Protein", "number":"AB316894-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ferritin Light Chain Capture Antibody", "number":"AB316894-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB316894-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316894-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316894-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ferritin Light Chain Detector Antibody", "number":"AB316894-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed : 24695223).
See full target information FTL
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com