Human Fetuin-A ELISA Kit
- Recombinant
- SimpleStep
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Human Fetuin-A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Fetuin-A with a sensitivity of 116.24 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
FETUA, PRO2743, AHSG, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A
- sELISA
Supplier Data
Sandwich ELISA - Human Fetuin-A ELISA Kit (AB269372)
Human MMP3 standard curve comparison.
Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab185989) and current human MMP3 SimpleStep ELISA kit (ab269372).
- sELISA
Supplier Data
Sandwich ELISA - Human Fetuin-A ELISA Kit (AB269372)
Interpolated concentrations of native Fetuin-A in human serum, plasma and cell culture supernatant samples.
The concentrations of Fetuin-A were measured in duplicates, interpolated from the Fetuin-A standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 20,000, plasma (citrate) 1 : 20,000, plasma (EDTA) 1 : 20,000, and HepG2 supernatant 1 : 600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fetuin-A concentration was determined to be 194.15 μg/mL in serum, 164.51 μg/mL in plasma (citrate), 172.44 μg/ml in plasma (EDTA), and 3.85 μg/mL HepG2 supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human Fetuin-A ELISA Kit (AB269372)
Example of human Fetuin-A standard curve in Sample Diluent 25BS.
The Fetuin-A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Fetuin-A ELISA Kit (AB269372)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fetuin-A concentration was determined to be 170.03 μg/mL with a range of 136.89 – 246.74 μg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Fetuin-A ELISA Kit (AB269372)
Human Fetuin-A standard curve comparison.
Standard Curve comparison between human Fetuin-A SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Reactivity data
Product details
Human Fetuin-A ELISA Kit (ab269372) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fetuin-A protein in cell culture supernatant, edta plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fetuin-A with 116.24 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Fetuin A is a highly abundant protein secreted by the liver that is capable of binding a wide variety of ligands, similar to serum albumin. A member of the cystatin family, Fetuin A plays a vital role in preventing tissue calcification and is also involved in fatty acid transport, insulin regulation and anti-inflammatory responses. Fetuin A is encoded by a single gene on chromosome 3, contains two cystatin domains, and exists as a heterodimer in its posttranslational form. Fetuin A is more abundant than serum albumin in fetal serum.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AHSG protein serves multiple functions including acting as an inhibitor of insulin receptor autophosphorylation and serine-threonine kinase activities. AHSG is part of a complex dynamic process involving calcium metabolism and bone mineralization. It binds to transforming growth factor-beta (TGF-β) and other proteins modulating cellular signals and maintaining homeostasis in tissues. The regulation of calcium ion balance by AHSG can protect against ectopic calcification preventing potential damage.
Pathways
Calcium signaling and the insulin signaling pathways integrate AHSG functions. AHSG interacts with proteins like insulin receptor and those in the canonical Wnt signaling pathway influencing glucose metabolism and bone development. The balance of its interactions in these pathways helps maintain metabolic homeostasis and skeletal integrity reflecting its versatile roles within complex networks.
Product protocols
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Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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